Morphogenic potential of cabbage cv. Pride of India, for multiple shoot induction was tested under in vitro conditions using cotyledon and hypocotyl explants. Aseptically grown seven to nine days old seedlings of cabbage were used as source of explants for reproducible plant regeneration studies. Forty different concentrations and combinations of TDZ (alone), TDZ with adenine, TDZ with NAA and ...

BACKGROUND AND AIMS Ilex khasiana is a rare and critically endangered holly endemic to the Khasi Hills of Meghalaya, India, and confined to a small number of pocket areas. In addition to conventional methods of propagation, endemic and threatened plants such as this could be more effectively multiplied and conserved using in vitro methods. Such techniques have the additional advantage of having...

To establish an efficient regeneration system for Anthurium andreanum cv Rubrun, seeds from plant spadixes were germinated on a medium supplemented with 2.2 μ M BA. After 2 weeks, 74% of the seeds germinated and four weeks later, micro-cuttings from these plantlets were subcultured on a medium containing 4.4 μ M BA and 0.05 μ M NAA. On average, 3.6 shoots per explant were obtained. Four weeks o...

We used in vitro callus and shoot cultures as target material for genetic transformation of silver birch (Betula pendula Roth) by particle bombardment. Cultivation of in vitro shoot cultures before particle bombardment and a long selection period, combined with a high concentration of selective agent after bombardment, led to the production of transformed plantlets that were stable, and no esca...

Environmental regulation of growth and dormancy of four Sorbus genotypes was studied in controlled environments. Emphasis was placed on assessment of the presence and nature of the deficient photoperiodic dormancy regulation system that has previously been reported for some woody Rosaceae species. Two genotypes of Sorbus aucuparia L. maintained indeterminate growth for 8 weeks and 9 weeks at te...

There are many benefits to growing Arabidopsis in solution-based media, especially when large amounts of root tissue are required for molecular and biochemical studies. Roots grown in soil are brittle and tend to break easily when removed from their substrate. We have developed an axenic liquid culture system that simplifies growing large amounts of roots from intact plants. This technique cons...

Oil palm is one of the most economically valuable oil seed plants, but the expansion of plantations has been limited by availability of seedlings, as the conventional propagation is through seeds, which have low germination rates. One possible solution for the large-scale production is the use of somatic embryogenesis. The aim of this study was evaluate the effects auxins 2,4-D and picloram on ...

Western-blot analysis and protein kinase assays identified two Ca(2+)-dependent protein kinases (CDPKs) of 55 to 60 kD in soluble protein extracts of embryogenic cultures of sandalwood (Santalum album L.). However, these sandalwood CDPKs (swCDPKs) were absent in plantlets regenerated from somatic embryos. swCDPKs exhibited differential expression (monitored at the level of the protein) and acti...

Shoot organogenesis and plant establishment has been achieved for Balanites aegyptiaca (L.) Del. from excised root explants. Young root explants obtained from 4 weeks old aseptic seedlings were used for the induction of direct shoot regeneration by incorporating various cytokinins either singly or in combination with auxins in Murashige and Skoog (MS) basal medium. Root explants cultured on MS ...

This study was carried out to evaluate the effects of salicylic acid in Marfona potato cultivar (Solanum tuberosum L.) for reducing the pathogenesis of Rizoctonia solani in potato tubers under greenhouse conditions. The pots sterilized soils had been infected by R. solani (21B1 strain) two weeks before transplanting the potato plantlets. The seedlings were treated with 0 (control), 0.1, 0.2, 0....