The recombinant adeno-associated viral (rAAV) vector is one of the most effective vectors in gene therapy because its low immunogenicity, high transduction efficiency, broad tissue specificity, and long-term transgene expression ability. HEK293T cells are widely used to produce rAAV vectors, acquisition rAAV-producing essential processes for higher production. To acquire such cells, a method id...

DNA size markers are widely used to estimate the size of DNA samples on agarose or polyacrylamide gelelectrophoresis (PAGE). DNA markers can be prepared by mixing PCR products with definite sizes.Alternatively, they are prepared by restriction enzyme digestion of the genomic DNA of bacteriophages ornatural and synthetic DNA plasmids. The present study describes engineering of ...

objective: in this study, two conserved genes (m1 and np) of influenza virus were expressed in a bicistronic vector in order to develop a universal gene based vaccine. materials and methods: plasmids m1-pires2-egfp, pires2-np were constructed by cloning the pcr products of m1 and np genes which were amplified from the a/peurto rico/8/34 (h1n1) influenza virus strain into the plasmid expression...

Background: DNA vaccines are third generation vaccines which have made promises to combat infectious diseases. Cationic liposomes are used as effective delivery systems for DNA vaccines to generate stronger immunity. Objective: Encapsulation of pcDNA3.1+PA plasmid, encoding protective antigen (PA) of Bacillus anthracis (B. anthracis) into cationic liposomes, and evaluation of its effect on spec...

Background: DNA vaccination with plasmid encoding bacterial, viral, and parasitic immunogens has been shown to be an attractive method to induce efficient immune responses. Bacteria of the genus Brucella are facultative intracellular pathogens for which new and efficient vaccines are needed. Methods: To evaluate the use of a DNA immunization strategy for protection against brucellosis, a pla...

background type 1 diabetes mellitus is one of the metabolic diseases that cause insulin-producing pancreatic ß cells be destroyed by immune system self-reactive t cells. recent­ly, new treatment methods have been developed including use of the stem cells, ß islet cells transplantation and gene therapy by viral and non-viral gene constructs. objectives the aim of this project was preparing the n...

introduction: cervical cancer is the second most common cancer in women worldwide and the role of human papillomavirus (hpv) has been proved in its etiology. the currentley available l1-capsid-protein-based vaccine is highly immunogenic and very high titers of serum antibodies can be obtained by its injection, but unfortunately it is restricted to only a few hpv genotypes and is relatively expe...

The segregation of plasmids in a bacterial population is investigated. Hereby, a dynamical model is formulated in terms of a size-structured population using a hyperbolic partial differential equation incorporating non-local terms (the fragmentation equation). For a large class of parameter functions this PDE can be re-written as an infinite system of ordinary differential equations for the mom...

Biological units such as macromolecules, organelles, and cells are directed to a proper location by gradients of chemicals. We consider a macroscopic element with surface binding sites where chemical adsorption reactions can occur and show that a thermodynamic force generated by chemical gradients acts on the element. By assuming local equilibrium and adopting the grand potential used in thermo...