We attempted to study the possible relationships between neutrophil-type procollagenase/pro-matrix metalloproteinase (MMP-8) and the serine proteinases plasmin, cathepsin G and tryptase in bronchiectasis. The presence of the plasmin/plasminogen system and plasmin-, cathepsin G- and tryptase-like activities were compared to the activity of endogenously activated MMP-8 in bronchoalveolar lavage (...

OBJECTIVE Plasmin activates several proinflammatory pathways at the cellular level in vitro. Lipopolysaccharide (LPS) administration to healthy humans results in a rapid generation of plasmin activity, accompanied by activation of a number of inflammatory systems. METHODS AND RESULTS To determine the role of early plasmin activity in LPS-induced inflammation in vivo, 16 healthy males received...

PURPOSE The mechanisms that trigger ganglion cell death in ischemic retinal diseases are not clearly understood. Using a mouse optic nerve ligation model, the objective of this study was to test the hypothesis that extracellular matrix (ECM) modulating plasminogen activators (PAs) potentiate ganglion cell loss. METHODS Optic nerve ligation was performed to initiate ganglion cell loss in the r...

BACKGROUND AND OBJECTIVES Hypervolemia is a common feature of patients with CKD and associated with hypertension. Recent work has shown stimulation of sodium retention by urinary plasmin during nephrotic syndrome. However, it is unclear whether plasminuria plays a role in patients with stable CKD and non-nephrotic proteinuria. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS In this cross-sectio...

Inhibitory effects of nine carbocyclic DNA minor groove binders on amidolytic activities of plasmin, trypsin and urokinase were examined. Some of the studied compounds affected plasmin or trypsin activity, but not urokinase activity. One of the pentamidine analogues (5) and two bis-netropsin like compounds (6, 8) were potent inhibitors of plasmin (IC50 equals 90 and 100 microM), whereas an anal...

Proteolytic activation of the epithelial sodium channel (ENaC) involves cleavage of its γ subunit in a critical region targeted by several proteases. Our aim was to identify cleavage sites in this region that are functionally important for activation of human ENaC by plasmin and chymotrypsin. Sequence alignment revealed a putative plasmin cleavage site in human γENaC (K189) that corresponds to ...

Macrophage activation syndrome (MAS) is a life-threatening complication associated with systemic juvenile idiopathic arthritis or with adult-onset Still disease. This syndrome has several attributes common to hemophagocytic lymphohistiocytosis (HLH), because both of these life-threatening conditions are characterized by a supraphysiological elevation of cytokines, an increase in inflammation, m...

Edicine is a therapeutic agent in the management of thromboembolic blockade. Streptokinase presently utilized clinical mase and an extracellular protein secreted by certain strains beta-hemolytic streptococci. It activates plasminogen to produce plasmin, enzyme that breaks down fibrin clots through specific lysine binding sites.

Tillett and Garner (1) discovered that an extracellular protein elaborated by certain strains of streptococci produces the lysis of clots which contain human fibrinogen or thrombin. M&tone demonstrated that the bacterial product was not in itself fibrinolytic but interacted with a lytic material in human plasma (2). The situation was greatly clarified and a rational nomenclature introduced by C...

A comparison was made of the esterase and activator activities of the various activated forms of human plasminogen and their streptokinase complexes with Nalpha-Cbz-L-lysine-p-nitrophenyl ester as the substrate. The steady state kinetic properties of Glu- and Lys-plasmins, and Glu- and Lys-plasminogen-streptokinase complexes were identical, while the Lys-plasmin-streptokinase complex showed a 2...