Pyrrolobenzodiazepines (PBDs) are covalent-binding DNA-interactive agents with growing importance as payloads in Antibody Drug Conjugates (ADCs). Until now, PBDs were thought to covalently bond to C2-NH2 groups of guanines in the DNA-minor groove across a three-base-pair recognition sequence. Using HPLC/MS methodology with designed hairpin and duplex oligonucleotides, we have now demonstrated t...

Defensins are small antimicrobial peptides (AMPs) that play an important role in the innate immune system of mammals. Since the effect of mycotoxin contamination of food and feed on the secretion of intestinal AMPs is poorly understood, the aim of this study was to elucidate the individual and combined effects of four common Fusarium toxins, deoxynivalenol (DON), nivalenol (NIV), zearalenone (Z...

Polo-like kinase 1 (Plk1) plays several roles in cell division and it is a recognized cancer drug target. Plk1 levels are elevated in cancer and several types of cancer cells are hypersensitive to Plk1 inhibition. Small molecule inhibitors of the kinase domain (KD) of Plk1 have been developed. Their selectivity is limited, which likely contributes to their toxicity. Polo-like kinases are charac...

OBJECTIVE To generate recombinant Bacillus subtilis (B. subtilis) engineered for expression of porcine β-defensin-2 (pBD-2) and cecropin P1 (CP1) fusion antimicrobial peptide and investigate their anti-bacterial activity in vitro and their growth-promoting and disease resisting activity in vivo. METHODS The pBD-2 and CP1 fused gene was synthesized using the main codons of B. subtilis and inse...

Programming-by-demonstration (PBD) is a new paradigm for programming industrial robots enabled by the development of the DLR/KUKA light-weight robot. Although the PBD approach facilitates and simplifies the generation of robot programs, the technician still needs to have skills and knowledge about the robotic system in order to produce efficient trajectories and to exploit the abilities of the ...

Introduction Cosmetic and plastic surgery procedures, such as liposuction, rhinoplasty (nose reshaping), and breast augmentation, and minimally invasive procedures, such as Botox injections and chemical peels, continue to increase in popularity in the West (American Society of Plastic Surgeons 2014). According to the American Society of Plastic Surgeons (ASPS), the world’s largest organization ...

Small bowel or colon loop stomy is performed in case of surgery, due to mechanical ileus or when there is risk of leakage below the anastomosis. Loop stomy is usually a temporary stoma, although in selected cases it might become definitive. It is assumed that each stoma should be supplied in stomy equipment during the surgical porocedure. In most centers, the most common method to maintain the ...

This paper describes the technological process for waste IC components reuse and studies its effect on the reliability of plastic IC components through some experiments. Results show that the high temperature thermal shock during printed circuit board (PCB) disassembly and appearance repair could lead to interfacial delamination and subsequent popcorn cracking in plastic IC packages. In the end...

The definition of “plastic surgery” has been subject to a variety of interpretations. To the practitioner who has completed formal training in plastic surgery, it is defined and inherently recognized as a specialty that encompasses a vast body of knowledge and that requires a comprehension of fundamental principles and concepts that allows one to plan and execute a particular operation. In othe...