BACKGROUND Abnormal adhesiveness of red blood cells to endothelium has been implicated in vaso-occlusive crisis of sickle cell disease. The present study examined whether the SAD mouse model exhibits the same abnormalities of red blood cell adhesion as those found in human sickle cell disease. DESIGN AND METHODS The repertoire of adhesive molecules on murine erythrocytes and bEnd.3 microvascu...

BACKGROUND Trafficking of transplanted endothelial progenitor cells (EPCs) to an ischemic organ is a critical step in neovascularization. This study was performed to elucidate the molecular mechanism of EPC trafficking in terms of adhesion molecules. METHODS AND RESULTS Using murine hindlimb ischemia model, we examined expressions of E-selectin, intercellular adhesion molecule-1 (ICAM-1), vas...

Endothelial cells synthesize a heterodimeric adhesion molecule. the vitronectin receptor (VnR). which is similar to the platelet glycoprotein (GP)llb/IlIa complex. The subunits of the endothelial VnR (VnRa and GPIIIa) have been studied for their ability to express alloantigens associated with platelet GPIIb and lIla. We previously showed that endothehal GPIIIa can express the platelet alloantig...

Intercellular adhesion molecule (ICAM)-1 plays a vital role in the process of leukocyte transmigration through endothelial cell (EC) barriers and has been shown to mediate signal transduction events in ECs induced either by its cross-linking or by the binding of T lymphocytes. Immunoblotting of ICAM-1 of Triton X-100 detergent fractions demonstrated that the majority of ICAM-1 was contained wit...

objective(s): fluvoxamine is a well-known selective serotonin reuptake inhibitor (ssri); despite its anti-inflammatory effect, little is known about the precise mechanisms involved. in our previous work, we found that ip administration of fluvoxamine produced a noticeable anti-inflammatory effect in carrageenan-induced paw edema in rats. in this study, we aimed to evaluate the effect of fluvoxa...

Platelet/endothelial cell adhesion molecule-1 (PECAM-1, CD31), a 130-kDa glycoprotein member of the Ig superfamily of transmembrane proteins, is expressed on endothelial cells, platelets, and subsets of leukocytes. It functions as a cell adhesion molecule as well as a scaffolding molecule capable of modulating cellular signaling pathways. In this study, using PECAM-1-deficient (KO) mice, as wel...

Disruption of endothelial cell function is a principle event in cardiovascular disease. Accordingly, therapies have mostly focused on repairing the endothelium, but little attention has been paid to the reconstruction of glycocalyx, which covers the endothelium and protects the function of endothelial cells. Sulodexide has a similar glycosaminoglycan structure to glycocalyx, so it is assumed to...

Platelet endothelial cell adhesion molecule-1 (PECAM-1) is a 130-kDa member of the immunoglobulin superfamily of cell adhesion molecules. It is characterised by the presence of two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within its cytoplasmic domain that led to its recent assignment to the Ig-ITIM family of inhibitory receptors. PECAM-1 is expressed on the surface of circulatin...

Leukocyte adhesion to endothelial cells plays a key role in inflammatory processes associated with end-organ injury. Endothelin-1 (ET-1), which stimulates inflammatory processes, contributes to cardiovascular damage in deoxycorticosterone (DOCA)-salt hypertension. We investigated whether ETA receptor blockade modulates in vivo leukocyte-endothelial cell interactions and expression of cell adhes...