We have determined the effect of glutathione (GSH) depletion on the cytotoxicity of three nitrogen mustards, six platinum complexes, and mitomycin C in a human ovarian carcinoma cell line. GSH levels in COLO 316 cells were depleted by exposure of cell monolayers to 0.5 mM D,L-buthionine-S,R-sulfoximine. GSH depletion significantly potentiated the cytotoxicity of L-phenylalanine mustard, chloram...

Platinum complexes of amino acids and peptides [1 – 5] have found interest as potential antitumor drugs with the idea that the coordinated bioligands might favor the transport and/or the selective uptake of the cytotoxic platinum unit into tumor cells [6 – 8]. Complexes of the type [Cl2Pt(N,Oamino acid anion)]− are formed from K2[PtCl4] and amino acids and have been reported as early as 1912 by...

Metal—Polyaniline nanocomposites such as Platinum-Polyaniline nanocomposite is prepared by insitu oxidative polymerization of aniline and reduction of Pt+4 ions into Pt nanoparticles. Thepolymerization of aniline was carried out in the presence of 1(2PtCN6 [Potassium Hexa CyanoPlatinate (IV)] as oxidizing agent. During the reaction aniline monomers undergo oxidation andform polyaniline (PANT) w...

The reaction of D-mannose and D-allose with [PtMe(3)(Me(2)CO)(3)]BF(4) 1 in acetone affords complexes [PtMe(3)L]BF(4) 5 and 6 (5, L = alpha-D-mannofuranose; 6, L = beta-D-allofuranose). The coordination mode and conformation of the carbohydrate ligands in 5 and 6 in acetone-d(6) have been determined from an analysis of J(HH), J(CH), and J(CC) in complexes formed using site-specific (13)C-labele...

Although Chk1 kinase inhibitors are currently under clinical investigation as effective cancer cell sensitizers to the cytotoxic effects of numerous chemotherapeutics, there is still a considerable uncertainty regarding their role in modulation of anticancer potential of platinum-based drugs. Here we newly demonstrate the ability of one of the most specific Chk1 inhibitors, SCH900776 (MK-8776),...

Cisplatin, cis-Diamminedichloroplatinum (II), is one of the most effective anticancer agents (Rosenberg, 1969). It has demonstrated a remarkable chemotherapeutic potential in a large variety of human solid cancers, such as, testicular, ovarian, bladder, lung and stomach carcinomas (Wong and Giandomenico, 1999; Guo and Sadler, 2000). The successful use of platinum (II) complexes as potent antica...

We have determined the effect of glutathione (GSH) depletion on the cytotoxicity of three nitrogen mustards, six platinum complexes, and mitomycin C in a human ovarian carcinoma cell line. GSH levels in COLO 316 cells were depleted by exposure of cell monolayers to 0.5 mM o,L-buthionine-S,fl-sulfoximine. GSH depletion significantly potentiated the cytotoxicity of Lphenylalanine mustard, chloram...