The distribution of newly synthesized proteolipid protein (PLP, 23 kdaltons) and myelin basic proteins (MBPs, 14-21.5 kdaltons) was determined in microsomal and myelin fractions prepared from the brainstems o1 10-30 d-old rats sacrificed at different times after an intracranial injection of 35S-methionine. Labeled MBPs were found in the myelin fraction 2 min after the injection, whereas PLP app...

Phantom Limb Pain (PLP) is an excruciating type of pain that gives a sensation of the continued presence of an amputated limb. Physicians have used many methods and drugs to treat PLP. We present a case of continued sciatic nerve block performed for PLP that developed after a traumatic amputation. We conclude that infusing a local anesthetic via a perineural sciatic catheter effectively treated...

The plasma pyridoxal-5'-phosphate (PLP) level of alcoholic subjects has been compared with that of non-alcoholic individuals in order to ascertain the incidence of abnormal vitamin B(6) metabolism in chronic alcohol abuse. 66 alcoholic subjects were selected on the basis that they did not exhibit abnormal liver function tests and hematologic findings. 35 of them had plasma PLP concentrations le...

Plasma levels of pyridoxal-5'-phosphate (PLP), the active coenzyme form of vitamin B6, were found to be significantly lower than normal in 22 out of 31 patients with decompensated cirrhosis or subacute hepatic necrosis. There was no significant difference in plasma PLP levels between those with liver disease due to alcohol and those with other varieties. When intravenous supplements with pyrido...

Pyridoxal 5'-phosphate (PLP; vitamin B(6))-catalyzed reactions have been well studied, both on enzymes and in solution, due to the variety of important reactions this cofactor catalyzes in nitrogen metabolism. Three functional groups are central to PLP catalysis: the C4' aldehyde, the O3' phenol, and the N1 pyridine nitrogen. In the literature, the pyridine nitrogen has traditionally been assum...

The performance of the PLP, log-RASTA-PLP, and J-RASTA-PLP front ends for recognition of highly reverberant speech is measured and compared with the performance of humans and the performance of an experimental RASTA-like front end on reverberant speech, and with the performance of a PLP-based recognizer trained on reverberant speech. While humans are able to reliably recognize the reverberant t...

An Overview on PLP-dependent Enzymes Pyridoxal 5’-phosphate (PLP), the biologically active of vitamin B6, was first identified in the mid-forties as the cofactor for the transamination reaction. Since then, PLP-dependent enzymes have been the focus of extensive biochemical research. The interest aroused by these enzymes is due to their unrivalled catalytic versatility and their widespread invol...

Markedly increased circulating concentrations of pyridoxal-5'-phosphate (PLP) were found in each of 14 patients representing all clinical forms of hypophosphatasia, an inborn error characterized by deficient activity of the tissue nonspecific (bone/liver/kidney) isoenzyme of alkaline phosphatase (AP). The mean PLP concentration in plasma was 1174 nM (range, 214-3839 nM) in the patients and 57 +...

Glucose 6phosphate dehydrogenase from streptomyces aureofaciens was purified and inactivated by pyridoxal 5′-phosphate (PLP). The inactivation was a pseudo-first order and time-dependent reaction. Complete inactivation was achieved at 0.2mM PLP within 16 minutes. The type of inhibition was competitive with respect to Glucose 6phosphate. Spectral characteristics of PLP-enzyme complex corresponde...

We cloned and expressed human pyridoxal-5\'-phosphate (PLP) phosphatase, the coenzymatically active form of vitamin B6, in Escherichia coli using pET15b vector. Monoclonal antibodies (mAb) were generated against purified human brain PLP phosphatase in mice, and four antibodies recognizing different epitopes were obtained, one of which inhibited PLP phosphatase. The binding affinities of these f...