A series of electroactive aromatic polyamides with 4-(dimethylamino)triphenylamine [(NMe2)TPA] units in the backbone were prepared from a newly synthesized diamine monomer, 4,4′-diamino-4′′-(dimethylamino)triphenylamine, and various aromatic dicarboxylic acids via the phophorylation polyamidation reaction. These polyamides are readily soluble in many organic solvents and can be solution-cast in...

Pyrrole-imidazole (Py-Im) polyamides can bind to the predetermined base pairs in the minor groove of double-helical DNA with high affinity. These synthetic small molecules can interfere with transcription factor-DNA interaction and inhibit or activate the transcription of corresponding genes. In the present study, we designed and synthesized a Py-Im polyamide to target -545 to -539 base pairs o...

The cooperative assembly of multiprotein complexes results from allosteric modulations of DNA structure as well as direct intermolecular contacts between proteins. Such cooperative binding plays a critical role in imparting exquisite sequence specificity on the homeobox transcription factor (Hox) family of developmental transcription factors. A well-characterized example includes the interactio...

Three DNA binding polyamides () were synthesized that bind with high affinity (K(a) = 8.7. 10(9) m(-1) to 1.4. 10(10) m(-1)) to two 7-base pair sequences overlapping the Ets DNA binding site (EBS; GAGGAA) within the regulatory region of the HER2/neu proximal promoter. As measured by electrophoretic mobility shift assay, polyamides binding to flanking elements upstream () or downstream (2 and 3)...

We describe a photonic waveguide where FRET is routed uni-directionally along a double-stranded DNA track. The efficiency of FRET is modulated by the supramolecular control of fluorophores along double-stranded DNA using fluorophore-tethered Pyrrole-Imidazole polyamides (PAs). We show that uni-directional FRET is enhanced by the complete assembly of each of the constituent parts, resulting in t...

Thirteen polyamides were synthesized by polycondensation using phosphorylation, low temperature solution and interfacial solution methods from 2, 2’-Dichloro-4, 4’-diaminoazobenzene condensed with different aliphatic diacids. These were characterised by inherent viscosity, solubility and spectral studies like UV-visible and IR. Moisture regain and dyeing properties have also been studied. TGA a...

      In view of potential biological activities of small molecule polyamides, we synthesized some novel N-(2-aminoethyl)-1-benzyl-2- (alkylthio)-1H-imidazole-5-carboxamide (7a,b), and N-(2-(1-benzyl-2-(alkylthio) -1H-imidazole- 5-carboxamido) ethyl)-1-benzyl-2- (alkylthio)-1H-imidazole-5-carboxamides (8a,b) as antitumor agents. The antitumor activity of compounds 7a,b and 8a,b was studied at c...

A new upper limit of binding site size is defined for the hairpin polyamide-DNA motif. Ten-ring hairpin polyamides containing pyrrole (Py) and imidazole (Im) amino acids were designed for recognition of seven base pair (bp) sequences in the minor groove of DNA. The DNA binding properties of two polyamides, ImPyPyPyPyγ-ImPyPyPyPy-â-Dp, and ImImPyPyPy-γ-ImPyPyPyPy-â-Dp were analyzed by footprinti...

in view of potential biological activities of small molecule polyamides, we synthesized some novel n-(2-aminoethyl)-1-benzyl-2- (alkylthio)-1h-imidazole-5-carboxamide (7a,b), and n-(2-(1-benzyl-2-(alkylthio) -1h-imidazole- 5-carboxamido) ethyl)-1-benzyl-2- (alkylthio)-1h-imidazole-5-carboxamides (8a,b) as antitumor agents. the antitumor activity of compounds 7a,b and 8a,b was studied at concent...