fluorescent monitoring of dna amplification is the basis of real-time pcr. absolute quantification can be achieved using a standard curve method. the standard curve is constructed by amplifying known amounts of standards under identical conditions to that of the samples.the objective of the current study is to propose a mathematical model to assess the acceptability of pcr resulys.four commerci...

conclusions campylobacter is responsible for some important clinical problems such as enteritis and is also associated with meningitis and hemolytic-uremic syndrome. it is imperative to monitor the prevalence and antibiotic resistance of campylobacter spp. as well as other the zoonotic bacteria. results twelve (10%) campylobacter spp. from 120 stool samples were isolated including c. coli and c...

ornithobacterium rhinotracheale (ort) is a bacterium associated with respiratory disease, growth retardation, decreased egg production and mortality in chickens and turkeys. the objective of this study was isolation, identification and evaluation of antimicrobial susceptibility of ort bacterium in slaughtered broilers chicken flocks based on cultural and molecular tests in khuzestan province, s...

detection of genetically modified organisms (gmos) in food is an important issue for all the subjects involved in food control and customer’s right. due to the increasing number of gmos imported to iran during the past few years, it has become necessary to screen the products in order to determine the identity of the consumed daily foodstuffs. in this study, following the extraction of genomic ...

methods and materials: hepatitis b virus dna was detected by conventional pcr in some of the serum samples obtained from hbsag-positive chronic hepatitis b (chb) patients who were referred to health centers of shahrekord for routine monitoring of the disease. subsequently, using real-time pcr, the dna samples were used for genotyping and analysis of resistance to lamivudine. results: the dna wa...

background cryptosporidium spp. is a widespread protozoan parasite involving humans and animals. objectives this study was carried out to evaluate the immunofluorescence assay (ifa) and pcr results for more accurate diagnosis of faecal specimens. patients and methods forty six faecal human specimens of cryptosporidium oocysts were examined by pcr and ifa in calgary, canada. in statistical analy...

background toxoplasma gondii is an opportunistic parasitic organism causing infection in many mammals, including immunosuppressed patients. toxoplasmosis as an opportunistic infection is highly prevalent among patients receiving a kidney transplant. objectives the purpose of this study was to identify and determine the prevalence of toxoplasma gondii in clinical samples collected from patients ...

conclusions the high prevalence of oxa β-lactamase and high genetic diversity of p. aeruginosa indicated that the resistance of p. aeruginosa might be expanding in our studied hospitals. background pseudomonas aeruginosa is main bacterial pathogen accountable for nosocomial infections. furthermore, it could potentially become resistant to β-lactams, aminoglycosides and fluoroquinolones antibiot...