Background & Aims: In recent years, molecular methods for characterizing genetic heterogeneity have found a major place in modern approaches. In this study, two different molecular techniques including Restriction Fragment Length Polymorphism (RFLP) and Multi Locus microsatellite typing (MLMT) were carried out in order to evaluate genetic heterogeneity among isolates of Leishmania major in Iran...

Purpose: The aim of this study was to investigate whether there is a relationship between surfactant protein B (SFTPB) C1580T polymorphism and acute bronchiolitis.
 Materials Methods: analyzed the allele frequency genotype distribution for SFTPB using polymerase chain reaction-restriction fragment length (PCR-RFLP) technique in 103 bronchiolitis infants 102 healthy infants.
 Results: ...

more than 80% of the total milk protein contains caseins that are to forms α-s1, α-s2, β-casein and kappa casein. kappa casein is smaller than other milk proteins but due to have a role in size and stability of micelles. the present study describes polymorphism of kappa casein gene. this is the first study of kappa casein gene polymorphism in najdi cattle of iran. we used the polymerase chain r...

the genetic variation and population structure of narrow-clawed crayfish (astacus leptodactylus) was examined by means of polymerase chain reaction (pcr) restriction fragment length polymorphism (rflp) analysis of the cytochrome oxidase subunit i (coi) of mitochondrial dna. a total of 194 adult specimens were collected from seven sample sites including, two in the south caspian sea and one each...

The broad goal of this research was to examine the nature of the Melanocortin receptor 1 (MC1R) locus on the coat color phenotype of seven goat breeds with different color coat. Blood samples were collected from five Iranian indigenous (Khalkhal, Markhor, Naeini, Najdi and Tali) and two exotic (Cashmere and Saanen) goat breeds. Polymerase chain reaction restriction fragment length polymorphism ...

conclusions the results revealed that the strains under study could be epidemically related. it seems that an alternative subtyping method is needed to study the relationship among clinical s. sonnei strains and their transmission. here, we reported for the first time, two strains of s. sonnei with a different pcr-rflp pattern for ipah gene. results all shigella isolates were positive for both ...