Extracts of defatted cocoa inhibited the biosynthesis of extracellular polysaccharide by both cell-free and cell-associated streptococcal glucosyltransferases, but did not affect growth or acid production. Both water-insoluble and water-soluble polysaccharide syntheses were inhibited, and the net result was a shift away from adherence-supportive polysaccharide. Inhibition was non-competitive wi...

The formation of cellular polysaccharide by cells of the genus Brucella has been reported by several investigators: Favilli and Biancalani (1934), Higginbotham and Heathman (1936), and Libby and Joyner (1941). These workers, however, dealt mainly with the immunological characteristics of the polysaccharide, and little emphasis was placed upon the nutritional conditions affecting its production....

A high molecular weight heteropolysaccharide, composed of glucose, glucuronic acid, N-acetylglucosamine, and mannose in an approximate molar ratio of 1:2:2:5, respectively, was isolated from phage K-2 and from the soluble fraction of phage-infected Aerobacter aerogenes lysates. Treatment of pure phage with 8 M urea at 4 degrees quantitatively solubilizes the bound polysaccharide and capsular po...

BACKGROUND Mushroom showed pellet, clump and/or filamentous mycelial morphologies during submerged fermentation. Addition of microparticles including Talc (magnesium silicate), aluminum oxide and titanium oxide could control mycelial morphologies to improve mycelia growth and secondary metabolites production. Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermen...

Caulobacter crescentus cells adhere to surfaces by using an extremely strong polar adhesin called the holdfast. The polysaccharide component of the holdfast is comprised in part of oligomers of N-acetylglucosamine. The genes involved in the export of the holdfast polysaccharide and the anchoring of the holdfast to the cell were previously discovered. In this study, we identified a cluster of po...

background :  kefir contains lactic acid bacteria ( lactobacillus , lactococcus , leuconostoc , acetobacter and streptococcus ) and yeasts ( kluyveromyces , torula , candida , and saccharomyces ). kefiran is the polysaccharide produced by lactic acid bacteria in kefir.   methods :  kefiran was prepared from milk containing 0.5% fat and 10 grams kefir grains and was separated from kefir by ethan...

24 Caulobacter crescentus cells adhere to surfaces using an extremely strong polar 25 adhesin called the holdfast. The polysaccharide component of the holdfast is 26 comprised in part of oligomers of N-acetylglucosamine. The genes involved in the 27 export of the holdfast polysaccharide and anchoring of the holdfast to the cell were 28 previously discovered. In this study, we identify a cluster...

Myxococcus xanthus fibrils are cell surface-associated structures composed of roughly equal amounts of polysaccharide and protein. The level of M. xanthus polysaccharide production under different conditions in the wild type and in several mutants known to have alterations in fibril production was investigated. Wild-type exopolysaccharide increased significantly as cells entered the stationary ...

The Escherichia coli K5 capsular polysaccharide (glycosaminoglycan) chains are composed of the repeated disaccharide structure: -GlcAbeta1,4-GlcNAcalpha1,4-(where GlcA is glucuronic acid and GlcNAc is N-acetyl-D-glucosamine). The GlcA, present in most glycosaminoglycans, is donated from UDP-GlcA, which, in turn, is generated from UDP-glucose by the enzyme UDP-glucose dehydrogenase (UDPGDH). The...

In order to extract the polysaccharide B of brucella used in Radial Immunodiffusion test (RID) for detection of infected cattle, a quick, simple and safe method is proposed. It consists of using phenol killed cells of B. melitensis 16 M known to produce polysaccharide B. After autoclaving brucella cells resuspended in saline, the cells are separated by centrifugation and the polysaccharide B is...