Changes in cellular metabolism in response to pharmacological compounds can be detected using a biosensor known as a microphysiometer, which measures the rate at which cells release acidic metabolites. We have applied this technique to screen for effects of cation channel blockers on the metabolism of a variety of human and murine cell lines. At concentrations sufficient for cation channel bloc...

The respective and combined effects of sodium chloride, ascorbic acid, citric acid, potassium sorbate, and Thymus vulgaris extract on the growth of Aeromonas caviae and Aeromonas sobria were investigated. Sodium chloride (3%) significantly reduced the growth and 4% NaCl inhibited growth of the tested strains. Ascorbic acid (0. 1%), potassium sorbate (0.05%), and citric acid (0.03%) slightly inh...

KCNQ potassium channels are activated by changes in transmembrane voltage and play an important role in controlling electrical excitability. Human mutations of KCNQ2 and KCNQ3 potassium channel genes result in reduction or loss of channel activity and cause benign familial neonatal convulsions (BFNCs). Thus, small molecules capable of augmenting KCNQ currents are essential both for understandin...

A Simple high-performance thin layer chromatography (HPTLC) method for separation and quantitative analysis of losartan potassium, amlodipine, and hydrochlorothiazide in bulk and in pharmaceutical formulations has been established and validated. After extraction with methanol, sample and standard solutions were applied to silica gel plates and developed with chloroform : methanol : acetone : fo...

The effects of thiol compounds (L-cysteine ethyl ester, 2,3-dimercaptosuccinic acid, or 2,3-dimercapto-1-propanesulfonic acid) on the toxicity induced by chromate (potassium dichromate) were investigated in HeLa cells and mice. Chromate-induced cytotoxicity evaluated by inhibition of cell growth and chromium content of the cells was diminished by all of the thiol compounds tested when the cells...

The chemical form of the potassium promoter on an iron-alumina catalyst during ammonia synthesis has been studied by two methods, viz, (i) the measurement of the equilibrium constant of the process KNH, + He = KH + NH,, and (ii) chemical analysis of the used catalyst. The equilibrium constant measurements gave KTza = (12.9 k 0.5) X 10m3, AHl,,(KNH,) =-I 19 * 3 kJ mol-’ and S,O,,(KNH,) = 109 ? 4...

The biosynthesis of the mustard oil glucoside, benzylglucosinolate, was studied in Tropaeolum majus L. A number of labeled compounds were administered to plant shoots and the incorporation of tracer into benzylglucosinolate, isolated as the crystalline tetramethyl-ammonium salt, was measured. In order of decreasing efficiency of conversion into benzyl-glucosinolate the compounds fed were S-(bet...

The Palladium inserted hydridopalladium species represents as RCOOPdH. This work finds an interesting coupling of palladium RCOOPdH with potassium styryltrifluoroborates to synthesize new kinds styryl ester compounds. Pd(OAc)2 was most effective catalyst for this cross-coupling reaction under microwave irradiation and gave esters in good moderate yields.

A simple high-performance thin-layer chromatographic (HPTLC) method for separation and quantitative analysis of losartan potassium, atenolol, and hydrochlorothiazide in bulk and in pharmaceutical formulations has been established and validated. After extraction with methanol, sample and standard solutions were applied to prewashed silica gel plates and developed with toluene–methanol–triethylam...