due to the importance of production and propagation of virus-free potato plants, some of the factors involved in the propagation of potato (plantlet production and virus-free in vitro microtuberization) has been studied. in most reports, adding a mixture of amino acids play an important role in plant tissue culture and development of the explants in many cases increase. this experiment was cond...

Potato virus Y (PVY) is one of the most economically important plant pathogens. The PVY genome has a high degree of genetic variability and is also subject to recombination. New recombinants have been reported in many countries since the 1980s, but the origin of these recombinant strains and the physical and evolutionary mechanisms driving their emergence are not clear at the moment. The replic...

Verticillium wilt of potato is a major limiting factor in potato production caused by Verticillium dahliae. The objective of this study was to evaluate the efficacy two elicitors including Acibenzolar-S-methyl (ASM) and Chitosan as plant activator in controlling of potato verticillium wilt in vitro and greenhouse conditions. ASM and chitosan were tested in vitro using Potato dextrose Agar (PDA)...

The Pvr4 resistance gene in pepper confers a complete resistance to the three pathotypes of potato virus Y (PVY) and to pepper mottle virus (PepMoV). In order to use this gene in a marker-assisted selection (MAS) program and to permit the pyramiding of several potyvirus resistance genes in the same cultivar, tightly linked amplified fragment length polymorphism (AFLP) markers were obtained by t...

NUCLEAR FACTOR Y transcription factors belong to a multimember family and consist of NF-YA/B/C subunits. Members of the NF-Y family have been reported to regulate physiological processes in plant. In this study, we identified and annotated two NF-YA, nine NF-B, and twelve NF-YC proteins in the genome of Physcomitrella patens. Analyses of conserved domains demonstrated that PpNF-YA/B/C shared th...

ACADEMIC DISSERTATION To be presented, with the permission of the Faculty of Agriculture and Forestry of University of Helsinki, for public criticism in the auditorium Ls3 of building B (Latokartanonkaari 7, Helsinki), February 5 th , 2010, at 12 o' clock noon. List of Original Publications The following original publications are referred to using the bold roman numerals throughout the text. Po...

Tepfer, M. 1993. Viral genes and transgenic plants. What are potential environmental risks? Bio/Technology 11:1125–1132. Truve, E., A. Aaspollu, J. Honkanen, R. Puska, M. Mehto, A. Hassi, T.H. Teeri, M. Kelve, P. Seppanen, and M. Saarma. 1993. Transgenic potato plants expressing mammalian 2'-5' oligoadenylate synthetase are protected from potato virus X infection under field conditions. Bio/Tec...

Potato virus Y (PVY) is an important plant pathogen with a wide host range that includes, among others, potato, tobacco, tomato and pepper. The coat protein (CP) of PVY has been commonly used in phylogenetic studies for strain classification. In this study, we used a pool of 292 CP sequences from isolates collected worldwide. After detecting and removing recombinant sequences, we applied Bayesi...

A Reverse Transcription-Polymerase Chain Reaction (RT-PCR) technique was applied for the detection of Potato leaf roll polerovirus (PLRV) in dormant potato tubers. A primer pair was designed from the coat protein-encoding fragment of the PLRV genome that amplified a 336-bp product. The amplified product was detected in nucleic acid preparations from leaves and tubers of 5 cultivars and from pur...