BACKGROUND The need for effective parameters for selecting the best embryos is paramount when a large number of them are available for transfer. Other studies have reported that transfer of pre-selected embryos, based on cleavage to the 2-cell stage at 25 h and 27 h post-insemination/intracytoplasmic sperm injection (ICSI), increases implantation and pregnancy rates. We investigated whether ext...

SNARE-mediated membrane fusion is a pivotal event for a wide-variety of biological processes. SNAP-25, a neuron-specific SNARE protein, has been well-characterized and mouse embryos lacking Snap25 are viable. However, the phenotype of mice lacking SNAP-23, the ubiquitously expressed SNAP-25 homolog, remains unknown. To reveal the importance of SNAP-23 function in mouse development, we generated...

Abstract Understanding human peri-implantation development is necessary to improve in vitro fertilisation. Technical progress, such as scRNAseq and integrated stem cell models have dramatically improved our ability understand development. However, in-depth analysis systematic validation embryos remains build a reliable reference. Here, we started by integrating pre- post-implantation embryo dat...

Controlled ovarian hyperstimulation (COH) affects adversely implantation following in vitro fertilization and embryo transfer. COH with gonadotropins alters endometrial receptivity and luteal function. In addition periovulatory endometrial characteristics in stimulated cycles are considerably different compared with the natural cycles, and periovulatory secretory transformation is consistently ...

Embryos have evolved a remarkable capacity to find implantation site. The impressive navigation ability of natural blastocysts may rely on highly sensitive signals arising from embryos and specialized signal processing strategies in the endometrium. Navigation capabilities may be compromised in ICSI embryos because of altered biochemical signaling. The design and delivery of artificial blastocy...

Summary Understanding lineage specification during human pre-implantation development is a gateway to improving assisted reproductive technologies and stem cell research. Here we employ pseudotime analysis of single-cell RNA sequencing (scRNA-seq) data reconstruct early mouse embryo development. Using time-lapse imaging annotated embryos, provide an integrated, ordered, continuous tran...

The Transient Receptor Potential (TRP) channels are a family of cationic ion channels widely distributed in mammalian tissues. In general, the global genetic disruption of individual TRP channels result in phenotypes associated with impairment of a particular tissue and/or organ function. An exception is the genetic ablation of the TRP channel TRPM7, which results in early embryonic lethality. ...

An experimental work dealing with the gene modification using Cas9 RNA-based editing system was performed. Point site-specific breakpoints in gDNA were introduced at zygote stage by microinjection of spCas9 mRNA protein and guide RNAs into cytoplasm. Oocytes that extruded first second polar bodies used for injection. 2 series microinjections designs early bovine embryos made. The degeneration r...

I N the course of an investigation on the uptake of labelled ions by the rabbit embryo and its environment (Lutwak-Mann, Boursnell, & Bennett, 1960), experiments were done on pre-implantation blastocysts obtained from rabbits which had been treated parenterally with certain purine analogues. Histological examination of such blastocysts by the method of Moog & Lutwak-Mann (1958) showed that, as ...

Unilateral ovariectomy (ULO) and its consequences with endocrine replacement in pregnant mice are important to examine both follicular dynamics as well as the outcome of implantation and pregnancy. In mice, ovariectomy on fourth day morning (D4), before pre-implantation estrogen secretion induces delayed implantation and embryonic diapauses, i.e. a state of suspended animation of embryos. The p...