Introduction: Melatonin acts as an indirect antioxidant and is a powerful direct free radical scavenger and direct responses to melatonin in the gonads are detected. This study aims to investigate the influence of different doses of melatonin on preantral follicle development and oogenesis of in vitro cultured mouse ovarian follicles. Materials and Methods: Preantral follicles with diamet...

Evidence shows that luteinising hormone (LH) participates in enhancing the transition from early to antral stage of folliculogenesis. Functional LH receptor was demonstrated be expressed, albeit at a very low level, even smaller follicles during phase traditionally considered gonadotropin-independent suggesting role for accelerating rate progression non-growing and primary preantral/antral stag...

Background: In vitro maturation (IVM) of oocytes reduces the costs and averts the side-effects of gonadotropin stimulation for in vitro fertilization (IVF). Reliable IVM is an intellectual, scientific and clinical challenge with a number of potential applications. Objective: The effect of hCG was evaluated on the timing and regulation of in vitro ovulation for the Syrian mice oocytes in the pre...

An option for fertility preservation for women facing a cancer diagnosis involves the cryopreservation of ovarian tissue for later re-transplantation or in vitro culture, with in vitro culture preferred to avoid reintroduction of the cancer. Small, immature follicles survive the freeze-thaw process, and can be matured through in follicle maturation (IFM) that involves an initial growth of the f...

BACKGROUND We have previously shown that suspension culture prevents follicle flattening and maintains three-dimensional follicle architecture better than culture on flat plates. However, many of the follicles cultured in suspension do eventually rupture, as basement membrane integrity is lost and the three-dimensional structure of the follicle is altered. Therefore, the objective of this study...

Background: The fibroblast growth factors (FGF-4) are a group of heparin-binding single chain polypeptides that play a pivotal role in development, cell growth, tissue repairing and transformation. The aim of this study is to improve development and maturation outcome of vitrified mouse pre-antral follicle by adding of fibroblast growth factor (FGF) into medium culture. Materials and Methods: P...

The aim of the present study was to evaluate the effects of indole-3-acetic acid (IAA) on survival, activation, and growth of caprine preantral follicles using histological and ultrastructural criteria. Pieces of caprine ovarian cortex were cultured for 1 or 5 days in Minimum Essential Medium (MEM control medium) supplemented with different concentrations of IAA (10, 20, 40, or 100 ng/ml). Smal...