association analysis is a useful method for evaluation of significant association between molecular marker and phenotype of trait. this study was performed to evaluate association between traits related with powdery mildew resistance and molecular markers. this investigation was performed using 77 barley genotypes and aflp markers. in phenotypic evaluation, reaction of seedlings to powdery mild...

1Division of AIDS, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892; ZDepartment of Molecular Biology, Washington University, St. Louis, Missouri 63110; 3Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814 PCR is a technology born of the modern molecular biology era. The enzyme used...

one of the best strategies to control bacterial wilt caused by ralstonia solanacearum (smith) is generally based on breeding resistant cultivars. the information obtained from the expression of plant defense genes will provide new insight for improving plant resistance against pathogens. this study was to identify inducible genes under defense no death (dnd) reaction of tobacco (nicotiana tabac...

three iranian native strains and three japanese commercial lines of the silkworm bombyx mori were analyzed using amplified fragment length polymorphism (aflp) markers. a set of ten psti/taqi primer combinations amplified a total of 322 bands out of which 251 (%78) were polymorphic. estimates of nei’s gene diversity for all loci in individual strains and commercial lines indicated a higher degre...

Therminator DNA polymerase is an efficient DNA-dependent TNA polymerase capable of polymerizing TNA oligomers of at least 80 nt in length. In order for Therminator to be useful for the in vitro selection of functional TNA sequences, its TNA synthesis fidelity must be high enough to preserve successful sequences. We used sequencing to examine the fidelity of Therminator-catalyzed TNA synthesis a...

Saprolegnia species are oomycetes (watermolds), which are fungal-like filamentous eukaryotes, that are closely related to chromophyte algae (Baldauf 2003), and are capable of infecting multiple hosts (Sarowar et al. 2013). The amplified fragment length polymorphism (AFLP) and sequence data of the internal transcribed spacer (ITS) methods were used to study the genetic diversity and relationship...

PREMISE OF THE STUDY Microsatellite markers were isolated in the rainforest tree Lophira alata (Ochnaceae), an important timber tree from Central Africa, and cross-amplified on its savannah counterpart, L. lanceolata. METHODS AND RESULTS From a microsatellite-enriched library sequenced on a 454 GS FLX platform, 13 primer combinations were identified. Amplification was optimized in two multipl...

gene was identified; its full-length cDNA was cloned from a library and sequenced. The cDNA is a novel member of the Ras-superfamily and was named Dexras1 due to its ability to be induced by dexamethasone. Experiments are in progress to characterize the role of Dexras1 in the pituitary and in other steroid-responsive tissues. Overall, differential display was extremely useful in identification ...

We characterized the first microsatellite loci in the white-dotted mosquito, Culex restuans, a critical early spring West Nile virus vector. An enrichment protocol yielded 960 positive clones of which we sequenced 300. We designed primers to amplify 29 unique di-, tri- and tetranucleotide microsatellites and chose 17 that amplified consistently across populations and were polymorphic. We develo...

gene was identified; its full-length cDNA was cloned from a library and sequenced. The cDNA is a novel member of the Ras-superfamily and was named Dexras1 due to its ability to be induced by dexamethasone. Experiments are in progress to characterize the role of Dexras1 in the pituitary and in other steroid-responsive tissues. Overall, differential display was extremely useful in identification ...