This paper reported the purification and kinetic parameters characterization of an alkaline protease produced from Bacillus subtilis through submerged fermentation process using rice husk as growth supporting substrate (by-product of rice industry) collected from Pearl Rice Mill Hafiz Abad, Faisalabad. Bacillus subtilis was cultured in fermentation medium under some pre-optimized growth conditi...

Yellowtail ascites virus (YAV) is an aquabirnavirus that causes ascites in yellowtail, a fish often used in sushi. Segment A of the YAV genome codes for a polyprotein (pVP2-VP4-VP3), where processing by its own VP4 protease yields the capsid protein precursor pVP2, the ribonucleoprotein-forming VP3, and free VP4. VP4 protease utilizes the rarely observed serine-lysine catalytic dyad mechanism. ...

The extracellular matrix components show specific distribution patterns within various structures of the umbilical cord, among which Wharton's jelly is especially collagen-rich tissue. Cathepsin L is a potent cysteine protease engaged in degradation of extracellular matrix proteins, including collagens. We evaluated the activity and expression of cathepsin L, and the inhibitory effect of cystei...

Rhizopus oligosporus was used in the present study for the production of protease enzyme under SSF. Sunflower meal was used as by-product of oil industry incorporated with organic salts was employed for the production of protease enzyme. The main purpose of the present was to study different parameters of protease productivity, its yields and to optimize basal fermentation conditions. The optim...

A protease produced by Vibrio mimicus was purified to apparent homogeneity by ammonium sulfate fractionation and successive column chromatography on Sephacryl S-100 and Mono Q Monobeads. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (PAGE) of the final preparation of the enzyme revealed the homogeneity of the purified enzyme. Conventional PAGE showed that the purified protease m...

This study was aimed at producing protease and lipase simultaneously on a common medium by Bacillus licheniformis VSG1, which was isolated from a tannery effluent. The effect of media composition with respect to protein source, lipid source and emulsifier on the production of protease and lipase was analysed. Both those enzymes were produced under optimized conditions like pH, temperature and i...

Docking and quantum-chemical methods have been used for screening of drug-like compounds from the own database Voronezh State University to find inhibitors SARS-CoV-2 main protease, an important enzyme coronavirus responsible COVID-19 pandemic. Using SOL program more than 42000 3D molecular structures were docked into active site 1000 ligands with most negative values score selected further pro...

Protease activity of actinomycetes isolated from various soil types of Georgia has been studied. Protease activity strains have been identified among mesophilic and extremophilic actinomycetes. Some strains of mesophilic, alkaliphilic and halophilic actinomycetes are characterized by alkaline protease activity. Their pH optimum for enzyme activity is 10-11. Strains of mesophilic actinomycetes: ...

Article history: Received on: 10/07/2012 Revised on: 03/08/2012 Accepted on:23/08/2012 Available online: 28/09/2012 Bacterial are well known for their ability to excrete enzymes into the environment. Bacillus sp. being industrially important organisms produces a wide variety of extra-cellular enzymes including proteases. Bacillus sp. isolated from local marine samples collected from Saudi Arabi...

An intracellular serine protease, which is believed to initiate the degradation of several intracellular pyridoxal phosphate-dependent enzymes, was localized by immunofluorescence in atypical mast cells of the lamina propria and in intraepithelial cells of the rat small intestine. Some mucus-secreting goblet cells also contained the protease antigen. Atypical mast cells containing the enzyme we...