Protein engineering approach enables effective immobilisation of thermostable affinity proteins onto polystyrene materials.

Glycosylation can be a promising tool for engineering protein-based biomaterials specifically regarding adhesion.

1 Targets and Tasks for Protein Engineering . . . . . . . . . . . . . . . . . . 56 1.1 Folding . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 1.1.1 Thermodynamic Stability . . . . . . . . . . . . . . . . . . . . . . . . . 57 1.1.2 Thermal and Environmental Stability . . . . . . . . . . . . . . . . . . . . 57 1.1.3 Other Folding Considerations . . . . . . . . . . . . . . . . . ...

The stability, size, and structure of avian pancreatic polypeptide (aPP) makes it a useful starting point for the design of miniature proteins that bind with high affinity and specificity to DNA1-4 and proteins5-14 and inhibit their interactions, both with high affinity and specificity, both in vitro1-6,8,9,11-14 and in mammalian cells and extracts.7,10 The utility of these molecules in a cellu...

Millions of novel protein sequences are currently being discovered, at an incredible pace. For instance, a recent article1 described the identifi cation of 8 complete and 789 draft genomes within a single metagenome library from an aquifer water sample. Unfortunately, the analysis of this huge pool of sequences remains challenging, given that the function of ~30–40% of the genes remains unknown...