Extracellular plant proteins are involved in numerous processes including nutrient acquisition, communication with other soil organisms, protection from pathogens, and resistance to disease and toxic metals. Insofar as these proteins are strategically positioned to play a role in resistance to environmental stress, biologists are interested in proteomic tools in analyzing extracellular proteins...

The localization of FtsI (PBP3), a penicillin-binding protein specifically required for cell division in Escherichia coli, was investigated by immunofluorescence microscopy and found to localize to the septum. The localization of FtsI was not observed in ftsZ or ftsA mutants, indicating that it was dependent on the prior localization of these proteins. Addition of furazlocillin, a specific inhi...

Like their eukaryotic counterparts, bacterial cells have a highly organized internal architecture. Here, we address the question of how proteins localize to particular sites in the cell and how they do so in a dynamic manner. We consider the underlying mechanisms that govern the positioning of proteins and protein complexes in the examples of the divisome, polar assemblies, cytoplasmic clusters...

Integral membrane proteins (IMPs) contain localization signals necessary for targeting to their resident subcellular compartments. To define signals that mediate localization to the Golgi complex, we have analyzed a resident IMP of the Saccharomyces cerevisiae Golgi complex, guanosine diphosphatase (GDPase). GDPase, which is necessary for Golgi-specific glycosylation reactions, is a type II IMP...

In recent years, wireless sensor networks have drawn great attention. This type of network is composed of a large number of sensor nodes which are able to sense, process and communicate. Besides, they are used in various fields such as emergency relief in disasters, monitoring the environment, military affairs and etc. Sensor nodes collect environmental data by using their sensors and send them...

Measurement of the correlation between sensor-protein expression, motility and environmental change is important for understanding the adaptation process of cells during their change of generation. We have developed a novel assay exploiting the on-chip cultivation system, which enabled us to observe the change of the localization of expressed sensor-protein and the motility for generations. Loc...

localization may be cell-cycle dependent. Mutations in FA patients represent an important resource for understanding both the function of the FA proteins and the molecular basis of the disease. For instance, the FA patient-derived FANCC-L554P mutant, which has a leucine to proline mutation at amino acid 554, fails to complement the mitomycin-C sensitivity of FA-C cell lines7 and is defective in...

A nine-month old male child presented with low-grade fever, loose stools and facial puffiness. Clinically patient was otherwise normal except for a firm liver on palpation. The laboratory tests revealed hypoproteinemia (both albumin and globulin) and iron deficiency anemia. Differential diagnosis considered were: 1. Nephrotic syndrome, 2. Cystic fibrosis (in view of recurrent diarrhea and respi...