Results By transcriptional profiling, a 21-gene signature was identified as differentially expressed between Blau and NHV samples. Consistent with constitutive activation of NOD2 signaling in Blau, 14 out of 21 of these genes were similarly regulated by NOD2 ligand stimulation in a NHV whole blood assay (P<0.0001). Plasma protein profiling identified a 7-protein signature specifically upregulat...

Graphical Abstract The cover picture shows a graphic depiction of Benjamin Cravatt's face overlayed with and framed by some the important components (chemical probes, biological systems, experimental results) activity-based protein profiling (ABPP) technologies that he has pioneered. As highlighted throughout this special issue, ABPP approaches allow studying large variety systems many differen...

Combinatorial and modular methods to synthesize small molecule modulators of protein activity have proven be powerful tools in the development new drug-like molecules. Over past decade, these methodologies been adapted toward utilization activity- affinity-based chemical probes, as well chemoproteomic profiling. In this review, we will discuss how like multicomponent reactions, DNA-encoded libr...

The study aims to identify the most responsible compound for antiinflammation activity from Mitragyna speciosa leaves. Seventeen compounds previously reported have been isolated leave were virtually screened against human 5-lipoxygenase protein and analyzed according their binding energies. native ligand used was arachidonic acid, mitragynine found be strongest (Pubchem ID: 3034396). In additio...

We conducted a study to identify circular RNAs associated with invasive non-functioning pituitary adenomas and chemoresistance, as well construct competitive endogenous RNA network. performed expression profiling of 10 specimens using sequencing identified 37 differentially expressed RNAs, which were validated real-time quantitative polymerase chain reaction. also detected 26 microRNAs 388 mess...

Activity-based protein profiling has emerged as a powerful tool for visualizing glycosidases in complex biological samples. Several configurational cyclophellitol isomers have been shown to display high selectivity as probes for glycosidases processing substrates featuring the same configuration. Here, a set of deoxygenated cyclophellitols are presented which enable inter-class profiling of β-g...

We present a methodology using in vivo crosslinking combined with HPLC-MS for the global analysis of endogenous protein complexes by protein correlation profiling. Formaldehyde crosslinked protein complexes were extracted with high yield using denaturing buffers that maintained complex solubility during chromatographic separation. We show this efficiently detects both integral membrane and memb...