Mammalian geranylgeranyltransferase type II (GGTase-II) is a 100-kDa heterodimer that catalyzes the transfer of two 20-carbon geranylgeranyl groups from geranylgeranyl pyrophosphate onto C-terminal cysteine residues of Rab GTPases. This modification is essential for the biological activity of Rab proteins. Geranylgeranylation can be performed in vitro using recombinant GGTase-II but so far larg...

1046-5928/$ see front matter 2009 Elsevier Inc. A doi:10.1016/j.pep.2009.09.016 * Corresponding author. Fax: +1 215 503 6795. E-mail address: [email protected] (M 1 Two authors contributed equally. 2 Abbreviations used: PA, protective antigen; ATR/C capillary morphogenesis protein 2; VWA, the von Wil fragment of human immunoglobulin. Mass vaccination against anthrax with existing vac...

  Background: The purity and correct folding of a recombinant protein is critical for any structural, biochemical and vaccine design studies. Plasmodium vivax Duffy binding protein-II is a leading vaccine candidate for vivax malaria. In the present study, the purification process of recombinant DBP-IX (a variant form of PvDBP-II) was optimized to achieve the highest yield and purity. Moreover, ...

Protein purification is an essential procedure in fields such as biochemistry, molecular biology, and biophysics. Acquiring target proteins with high quality and purity is still difficult, although several tag systems have been established for protein purification. Affinity tag systems are excellent because they possess high affinity and specificity for acquiring the target proteins. Neverthele...