Proteinase B (EC 3.4.22.9) was purified from commercial baker's yeast and from wild type strains of Saccharomyces cerevisiae and Saccharomyces carlsbergensis. For large scale purification a procedure was developed involving hydrophobic chromatography on octyl-Sepharose 4B and gel filtration on Sephadex G-100. A rapid purification of small amounts of proteinase B was achieved by affinity chromat...

Proteolytic activity in extracts of lymphatic tissue has been described in the older literature,11 gelatin, casein, and serum albumin having been used as substrates. More recently bovine tonsils were reported to contain a protease that cleaves serum albumin.7 Rothschild and Junqueirae found proteolytic activity in lymph node homogenates, using globin as substrate. In the present investigation, ...

2-fold. Lack of the vacuolar endoproteinases yscA and yscB reduces the protein degradation rate by about 30% under the conditions of canavanine-induced synthesis of false proteins (Table 2). The remaining protein degradation rate of 15% under starvation conditions, and of 6(r-70°h under growing conditions in mineral medium in strains defective in the two vacuolar endoproteinases yscA and yscB, ...

Erythrocyte CR1, a C3b/C4b-binding complement-regulatory protein, is sensitive to proteolysis in vitro. To test the hypothesis that in vivo erythrocyte CR1 reduction results from intravascular proteinase activities, we used enzyme-linked immunosorbent assays to measure gamma-crosslinked fibrin degradation products (D-dimers) as indicators of coagulation/fibrinolytic activity, and complexes of n...

Human adenovirus contains a virion-associated proteinase activity essential for the development of infectious virus. Maximal proteinase activity in vitro had been shown to require three viral components: the L3 23-kDa protein, an 11-amino acid cofactor (pVIc), and the viral DNA. Here, we present a quantitative purification procedure for a recombinant L3 23-kDa protein (recombinant endoproteinas...

The Agaricus bisporus serine proteinase 1 (SPR1) appears to be significant in both mycelial nutrition and senescence of the fruiting body. We report on the construction of an SPR promoter::green fluorescent protein (GFP) fusion cassette, pGreen_hph1_SPR_GFP, for the investigation of temporal and developmental expression of SPR1 in homobasidiomycetes and to determine how expression is linked to ...

In the present study, the 50% hydro ethanolic extract of different varieties of spp.) peel such as Nendran (Musa spp. Pachanadan AAA) was evaluated for membrane stabilization and proteinase inhibitory activity was assessed at a concentration of 0.1g/ml for all the varieties. The standards used in this study include aspirin and diclofenac sodium. Of the four var albumin denaturation (63.42%) and...