نتایج جستجو برای: protoplast fusion
تعداد نتایج: 120504 فیلتر نتایج به سال:
The rice pattern recognition receptor (PRR) XA21 confers race-specific resistance in leaf infection by bacterial blight Xathomonas oryzae pv. oryzae (Xoo), and was shown to be primarily localized to the endoplasmic reticulum (ER) when expressed with its native promoter or overexpressed in the protoplast. However, whether the protein is still ER-localization in the intact cell when overexpressed...
Protoplasts are cells without cell walls. In plants protoplasts are usually liberated by tissue digestion with cellulases and pectinases purified from wood-rotting fungi. To keep the plasma membrane from bursting during protoplast isolation, an isotonic or slightly hypertonic medium is used. Because of the lack of external constraints on the membrane, protoplasts are spherical (figure 1B). One ...
Cucumber plants play an important role in economical field in Egypt. But they are infected by plant parasitic nematodes that leading to losses cucumber yield. This study attempted to construct more efficient bacterial strains may be able to controlling nematodes, as well as, maintenance the soil fertility using molecular biology method via induced protoplast fusion between different strains of ...
Saccharomyces cerevisiae M30, a high ethanol producing flocculent strain, was hybridized with Zygosaccharomyces rouxii TISTR1750, a halotolerant non-flocculent strain, by polyethylene glycol induced protoplast fusion. The protoplast fusion of these two yeast genera occurred with frequency of 5.89X10-5. Three fusants, namely RM11, RM37 and RM58, were selected based on their high ethanol fermenta...
Transient transformation is simpler, more efficient and economical in analyzing protein subcellular localization than stable transformation. Fluorescent fusion proteins were often used in transient transformation to follow the in vivo behavior of proteins. Onion epidermis, which has large, living and transparent cells in a monolayer, is suitable to visualize fluorescent fusion proteins. The oft...
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