UNLABELLED The platelet-rich plasma (PRP) is an autologous biotherapy commonly used for its healing properties. Once activated, platelets released a real "cocktail" of growth factor and cytokines implied in numerous regenerative processes. However the impact of medical practices associated to PRP therapeutic use on platelets functionality remains poorly known. OBJECTIVES we evaluated the in v...

OBJECTIVES To investigate the in vitro effects of Sodium Tetradecyl Sulphate (STS) and Polidocanol (POL) on clotting tests, clotting factors, platelets and microparticles. MATERIALS AND METHODS Platelet rich (PRP) and platelet poor (PPP) plasmas were incubated with varying concentrations of STS and POL. Clotting tests, platelet/plasma turbidity, and microparticle studies were performed. Speci...

Platelet rich plasma (PRP) has been shown to clinically accelerate healing of both soft and hard tissues. As a result, it has gained increasing popularity. However, the clinical effectiveness of each type of PRP preparation method can vary in technique and efficiency, and current methods to evaluate the platelet concentration efficiency of PRP systems have several limitations. Therefore, the pu...

The production of inexpensive autologous canine platelet rich plasma (PRP) with no requirement for specific equipment may provide a relatively easy source of growth factors and cell-rich fractions for stimulating healing processes and regeneration. In dogs many different manual or semi-automated methods for PRP production are described achieving wide ranges of platelet concentration, but few st...

Purpose Herein we evaluated the effects of platelet concentrate (PC) and platelet-poor plasma (PPP) on bone repair using noncritical defects in calvaria rabbits compared them to presence TGF-β1 osteocalcin reparative sites. Methods Five 8.7 mm diameter were created 15 animals. Each defect was treated differently, autograft (ABG), ABG associated with PC (ABG + PC), PPP PPP), isolated PPP, blood ...

Although mechanical stabilisation has been a hallmark of orthopaedic surgical management, orthobiologics are now playing an increasing role. Platelet-rich plasma (PRP) is a volume of plasma fraction of autologous blood having platelet concentrations above baseline. The platelet alpha granules are rich in growth factors that play an essential role in tissue healing, such as transforming growth f...

We examined the proliferative behaviour in tertiary culture cells of human skin fibroblasts (HSF) as well as cells of the rat 3Y1 diploid fibroblast line placed on a plastic substratum in a nutrient-rich medium containing high concentrations of platelet-poor plasma (PPP). Autochthonous human PPP was used for the HSF cells and heat-treated (at 56 degrees C for 30 min) bovine PPP was used for the...

background: the purpose of this study was to create biomaterial scaffolds like platelet-rich plasma (prp) and platelet-rich fibrin (prf) containing stromal cell-derived factor-1 (sdf1) as a chemokine to induce hyaline cartilage regeneration of rabbit knee in a full thickness defect. methods: we created a full thickness defect in the trochlear groove of thirty-six bilateral knees of eighteen mat...

Platelet-rich plasma (PRP) has been clinically used as an easily prepared growth factor cocktail that can promote wound healing, angiogenesis, and tissue remodeling. However, the therapeutic effects of PRP are still controversial, due partly to the lack of optimized and standardized preparation protocols. We used whole blood (WB) samples to optimize the preparation protocols for PRP, white bloo...

The aim of this study was to evaluate the effects of standardized platelet-rich plasma (PRP) concentrates from 10 human donors on cellular behavior. The standardized PRPs used were fivefold average and fivefold maximum baseline values in whole blood. Both these standardized PRPs were characterized by determining platelet numbers and subsequently growth factor concentrations in activated PRPs, c...