activity and mRNA expression (NOX1, NOX4, p67, p47, p22). Inhibitors of AP-1 significantly diminished the Ang II or TNF-stimulated p22 promoter activity and protein level. Transient overexpression of c-Jun/c-Fos upregulated p22 promoter activity. Transcription factor pull-down assay and chromatin immunoprecipitation demonstrated the physical interaction of c-Jun protein with predicted AP-1–bind...

Granulin A (GRN A), a peptide with a molecular 6 kDa, is derived from proteolysis of progranulin (PGRN). Previous study in our laboratory has shown that GRN A is able to inhibit cancer cell growth significantly. In the present study, we confirmed that GRN A can bind to α-enolase (ENO1) specifically as analyzed using Pull-down/MS approaches. The interaction of GRN A with ENO1 was further confirm...

background and objectives: ziziphora clinopodioides is a perennial herb and grows widely in west and northwest of iran. the aerial parts are used as appetizer, carminative and antiseptic as well as for the treatment of medical conditions such as high blood pressure, asthma hyperhidrosis, palpitation and insomnia methods: the aerial parts of ziziphora clinopodioides lam. were extracted by ethano...

A new CMOS buffer without short-circuit power consumption is proposed. The gatedriving signal of the output pull-up (pull-down) transistor is fed back to the output pull-down (pull-up) transistor to get tri-state output momentarily, eliminating the short-circuit power consumption. The HSPICE simulation results verified the operation of the proposed buffer and showed the power-delay product is a...

BACKGROUND The chromogenic assay based on MTT bioreduction was adapted to Proteus mirabilis viability estimations. We primarily intended to use the assay for the evaluation of novel antimicrobial compounds, including structures with possible permeabilizing activity. Therefore, the influence of basic permeabilizing agents like Triton X-100 and EDTA upon the MTT assay was studied. METHODS 3-(4,...

Conventional Western blot based pull-down methods involve lengthy and laborious work and the results are generally not quantitative. Here, we report the imaging beads-retained prey (IBRP) assay that is rapid and quantitative in studying protein-protein interactions. In this assay, the bait is immobilized onto beads and the prey is fused with a fluorescence protein. The assay takes advantage of ...

The aim of this study was to compare four in vitro cytotoxicity assays and determine their ability to detect early cytotoxic events. Two hepatoma cell lines, namely HTC and HepG2 cells, were exposed to cadmium chloride (0-300 microM) for 3, 5 and 8 h. Following exposure to the toxic metal cytotoxicity was determined with the lactate dehydrogenase leakage assay (LDH), a protein assay, the neutra...

To gain an insight into the molecular mechanisms of gefitinib resistance in nonsmall cell lung cancer, we screened out long noncoding RNA related to through microarray data gefitinib-sensitive and resistant cells. lncRNA ZMIZ1-AS1 was significantly upregulated HCC827/GR cells by screening data. Further real-time-qPCR results were consistent with Cell viability assay flow cytometry showed that i...