A thermostable amylopullulanase producing Clostridium thermosulfurogenes SVM17 was selected for the study. Culture conditions for growth and enzyme production parameters were optimized. Enzyme synthesis occurred at temperatures between 50 and 70°C with an optimum of 60°C. The enzyme production was observed between pH 4.0 and 9.0 with an optimum of pH 7.5. Maximum growth and enzyme production wa...

Cassava starch, typically, has resistant starch type 3 (RS3) content of 2.4%. This paper shows that the RS3 yields can be substantially enhanced by debranching cassava starch using pullulanase followed by high pressure or cyclic high-pressure annealing. RS3 yield of 41.3% was obtained when annealing was carried out at 400MPa/60°C for 15 min, whereas it took nearly 8h to obtain the same yield un...

Type IV pili (T4P) and T2SS (Type II Secretion System) pseudopili are filaments extending beyond microbial surfaces, comprising homologous subunits called "pilins." In this paper, we presented a new approach to predict pseudo atomic models of pili combining ambiguous symmetric constraints with sparse distance information obtained from experiments and based neither on electronic microscope (EM) ...

O processo de hidrólise do amido da torta de mamona por ácido e pela combinação de a-amilase, glicoamilase e pululanase foi realizado, seguindo de etapa de fermentação. A hidrólise química resultou em 27.3 g L de açúcares com 33.4% de eficiência de hidrólise. O hidrolisado obtido foi fermentado originando 11 g L de etanol (Y P/S =0.48 g g). A melhor condição de hidrólise enzimática foi (por gra...

In addition to the previously identified 4-alpha-glucanotransferase gene mgtA and the alpha-amylase gene amyA of Thermotoga maritima strain MSB8 we have now isolated three further genes encoding amylolytic enzymes from a gene library of this ancestral bacterium. The genes code for the extremely thermostable enzymes pullulanase (pulA), maltodextrin phosphorylase (agpA) and alpha-glucosidase (agl...