We set-up a linear city model with duopoly upstream and downstream. There is a transportation cost when consumers buy from a retailer and when retailers buy from a wholesaler. All location and pricing decisions are endogenous. When the wholesalers choose their locations …rst, they locate closer to the center relative to the retailers, and relative to when they move simultaneously. Each wholesal...

The mammalian long-chain acyl-CoA thioesterase, the enzyme that catalyses the hydrolysis of acyl-CoAs to free fatty acids, contains two fused 4HBT (4-hydroxybenzoyl-CoA thioesterase) motifs. The C-terminal domain of the mouse long-chain acyl-CoA thioesterase (Acot7) has been expressed in bacteria and crystallized. The crystals were obtained by vapour diffusion using PEG 2000 MME as precipitant ...

The gene encoding 2,4-dihydroxy-hepta-2-ene-1,7-dioate (HHED) aldolase (HpcH; EC 4.1.2) from Escherichia coli C was cloned into the high-expression plasmid pProEx-HTa and overexpressed in E. coli BL21 (DE3). The 28 kDa enzyme was purified using immobilized metal-affinity and size-exclusion chromatography prior to crystallization. Crystals were obtained by the hanging-drop vapour-diffusion metho...

The complete error-in-variables-model (EVM) framework, consisting of both design of experiments and parameter estimation stages, is applied to the terpolymerization of 2-acrylamido-2-methylpropane sulfonic acid (AMPS, M1), acrylamide (AAm, M2) and acrylic acid (AAc, M3). This water-soluble terpolymer has potential for applications in enhanced oil recovery, but the associated terpolymerization k...

Model Independent Analysis (MIA), which employs statistical methods to reveal one-micron charge-induced centroid changes in pulse-by-pulse beam-position-monitor (BPM) measurements at the SLAC linac [1] has been extended to storage rings, and is here applied at PEP-II to analyze transverse motion. The beam is resonantly excited by a shaker in the horizontal and vertical directions and 2050 conse...

We verify all quadrupole strengths (both normal and skew) and determine sextupole feed-downs as well as all BPM gains and BPM cross-plane couplings by an SVDenhanced least-squares fitting of the phase advances (only those which are accurately determined) and the Green’s functions (specified by the local transfer matrix components R12, R34, R32, and R14). The phase advances and the Green’s funct...

The enzyme L-aminoacylase catalyses the hydrolysis of N-acyl-L-amino acids from peptides or proteins. The recombinant enzyme from the hyperthermophilic archaeon Thermococcus litoralis has been purified to homogeneity. This zinc-containing enzyme has been crystallized from ammonium sulfate using the sitting-drop vapour-diffusion method. The crystals diffract to 2.8 A resolution and belong to the...

In this work, which is related to the current European Parliament Regulation on restrictions affecting refrigeration, four new three-component refrigerants have been proposed; all were created using low Global Warming Potential(GWP) synthetic and natural refrigerants. The considered mixtures consisted of R32, R41, R161, R152a, R1234ze (E), R1234yf, R1243zf, RE170. These theoretically tested wit...

In this paper, the composition of binary nonazeotropic working fluids is explored from perspective improving OTEC-ORC efficiency, and turbine designed to accommodate with fluid. It found that using a R152a/R32 mixture as fluid significantly higher than thermal efficiency system pure NH3, optimal mixed determined be 85 : 15 2.8% 1.7%, respectively, by 35.7% 151.2% compared NH3 ORC. According flu...

In chemical engineering, surrogate functions are commonly used in modeling thermodynamic properties of pure compounds because they often more accurate than equations state. 2020, we presented a semi-empirical model describing the vapor density from reduced triple point temperature to about 0.97 with only one temperature-dependent term. However, many cases range up critical is necessary. Therefo...