The effects of gentamicin, lithium and combination of these drugs on ultrastructural morphology of rat submandibular glands were studied. Daily intraperitoneal injection of 40 mg/kg of gentamicin which causes chronic salivary gland toxicity, led to a marked decrease in gland's dry weight and total protein concentration but did not affect DNA content. Electron micrograph (EM) of submandibular ...

Obesity has been linked to several chronic diseases, including fatty liver disease, heart diabetes mellitus, hypertension, obstructive sleep apnea, and cancer. The purpose of the current study was determine how Orlistat affected renal toxicity, DNA damage, apoptosis that were brought on by obesity in normal obese female rats. There four groups made up a total 20 rats (G1, Control; G2, Orlistat;...

1. The influence of exogenous or activated DNA template on the DNA polymerase activity in the different types of intact nuclei from rat brain tissue was determined. The different amounts or physical state of the DNA template did not produce significant differences in the relative distribution of the DNA polymerase activity between the separate groups of nuclei. 2. The DNA polymerase activities,...

DNA methylation is crucial for gene regulation and maintenance of genomic stability. Rat has been a key model system in understanding mammalian systemic physiology, however detailed rat methylome remains uncharacterized till date. Here, we present the first high resolution methylome of rat liver generated using Methylated DNA immunoprecipitation and high throughput sequencing (MeDIP-Seq) approa...

In situ end-labeling (ISEL) identifies DNA fragmentation in apoptotic or necrotic nuclei in tissue sections. However, application of ISEL on human brain requires conservation of DNA integrity during the postmortem delay (PMD) and good accessibility of fragmented DNA after (prolonged) tissue fixation. We therefore investigated ISEL in relation to PMD and fixation in rat and human brain. Applicat...

We examined DNA fingerprints of the spontaneously hypertensive rat from Shimane Institute of Health Science, Izumo, Japan, including seven substrains that were separated in the early stages of the establishment of the stroke-prone spontaneously hypertensive rat, and compared their fingerprints with those of rats from other sources. Obtained DNA fingerprints revealed that, in both the stroke-res...

Ca depletion/Interphase death/Rat thymocytes Role of Ca ion in development of radiation damage of rat thymocytes was examined. Free DNA release, an expression of chromatin degradation, and the percentage of erythrosin B stained (dead) cells were increased after incubation of 1000 R X-irradiated thymocytes with normal Ca-containing medium. Incubation of the irradiated cells with Ca ion-depleted ...

Neonatal rat lens epithelium has a high 12(S)-hydroxyeicosatetraenoic acid [12(S)-HETE] synthetic capacity, which decreases as epithelial cell proliferation decreases with age. To determine whether products of the 12-lipoxygenase pathway are involved in lens cell proliferation, we measured the effect of 12-lipoxygenase inhibitors on endogenous 12-HETE production, epidermal growth factor/insulin...

A method is given for small-scale preparation of DNA from 1.0-1.5g of adult rat tissues. The product from brain or liver is characterized by base ratios and phosphorus content which accord with reported values for rat tissue. It is reasonably free of RNA, protein and glycogen. It contains 5-hydroxymethylcytosine at a content of about 15% of the total cytosine bases present. 5-Hydroxymethylcytos...

The in vivo metabolism and DMA binding of benzo(a)pyrene (BP) was examined in male Sprague-Dawley rat. BP was ad ministered i.v. at dose levels of 1.0 and 10.0 /¿mol/kg. DNA samples from [3H]BP-treated rats were enzymatically digested to deoxyribonucleosides and analyzed by high-pressure liquid chromatography. Several chromatographically distinct nucleoside-bound adducts were observed in lung ...