Amplicon sequencing has been the method of choice in many high-throughput DNA sequencing (HTS) applications. To date there has been a heavy focus on the means by which to analyse the burgeoning amount of data afforded by HTS. In contrast, there has been a distinct lack of attention paid to considerations surrounding the importance of sample preparation and the fidelity of library generation. No...

Metallic surfaces and particles can have dramatic effects on fluorescence, including localized excitation, increased quantum yields, increased photostability and increased distances for resonance energy transfer (RET), and directional emission. While all these effects have not yet been realized in a single system, metal-enhanced fluorescence promises to provide the next generation of high sensi...

OBJECTIVE To investigate if the addition of dexmedetomidine to meperidine in a patient-controlled analgesia (PCA) device would reduce postoperative meperidine consumption when compared with meperidine alone. METHODS Forty patients scheduled for elective abdominal surgery under general anesthesia in Suleyman Demirel University Medical School, Isparta, Turkey between February and September 2006...

We conducted field and laboratory experiments to evaluate whether treating pregnant bighorn ewes with a combination of an experimental Pasteurella trehalosi and Mannheimia haemolytica (formerly P. haemolytica) vaccine and a commercially-available bovine P. multocida and M. haemolytica vaccine would increase lamb survival following a pneumonia epidemic. Three free-ranging bighorn herds affected ...

Errors that are commonly made in the quantification of biogas from anaerobic digestion experiments were investigated. For liquid displacement gasometers where a barrier solution separates the biogas and the atmosphere, inaccuracy due to gas diffusion was examined experimentally. Acidified saturated saline solution was the most suitable barrier solution, as biogas characteristics changed least w...

The behavior of water molecules at the surface of nonionic surfactant (monomyristolein) and effects of monovalent ions on the behavior are investigated using the heterodyne-detected vibrational sum frequency generation spectroscopy. It is found that water molecules at the surface are oriented with their hydrogen atoms pointing to the bulk, and that the degree of orientation depends on the anion...

BACKGROUND People in pain, or expecting pain, sometimes bias their attention towards pain-relevant cues. Perhaps they also bias their attention towards the body part in question. AIM To determine if experimentally induced pain, and the expectation of pain, involve an information processing bias towards the hand in question. METHODS Seventeen asymptomatic subjects performed a hand laterality...

Although fluoride induces corrosion in titanium in an oral environment, the influence of protein on this corrosion remains to be clarified. The objective of this study was to investigate suppression of fluoride-induced corrosion of titanium where albumin was either present in a solution or where albumin was preadsorbed on titanium. Titanium dissolution in titanium specimens and surface characte...