S-nitrosoglutathione (GSNO) is considered a natural nitric oxide (NO.) reservoir and a reactive nitrogen intermediate in animal cells, but little is known about this molecule and its metabolism in plant systems. In this work, using pea plants as a model system, the presence of GSNO in collenchyma cells was demonstrated by an immunohistochemical method. When pea plants were grown with a toxic Cd...

Macrophages (Mphi) have been implicated in the suppression of lymphocyte function following thermal injury. Splenocytes isolated from C57BL/ 6NCR female mice 4-7 days after thermal injury displayed suppressed proliferative responses to Concanavalin A (ConA) and lipopolysaccharide (LPS) and high levels of reactive nitrogen intermediate (RNI) production. Inhibition of nitric oxide synthase activi...

Nitrogen Dioxide: Nitrogen dioxide (NO2) is one of a group of highly reactive gasses known as "oxides of nitrogen," or "nitrogen oxides (NOx)." Other nitrogen oxides include nitrous acid and nitric acid. While EPA’s National Ambient Air Quality Standard covers this entire group of NOx, NO2 is the component of greatest interest and the indicator for the larger group of nitrogen oxides. NO2 forms...

Mixed signals? The immunology field got a hint that NO might be a host product in 1985, when Michael Marletta and his graduate student Dennis Stuehr at the Massachusetts Institute of Technology found an ex cess of nitrate in the urine of bacterially infected mice (1). To find its source, the researchers focused on macrophages, immune cells that help defend against infections. Meanwhile, at the ...