Objective: Reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) is one of the time-saving, accurate, and cost-effective alternative methods to real-time polymerase chain reaction (RT-PCR). This study aimed identify robustness a colorimetric RT-LAMP assay kit that we developed, detecting SARS-COV-2 viral RNA within 30 minutes using primer set special N gene against RT-PCR, gold...

Background: Enterovirus (EV) infections are one of the most common causes of aseptic meningitis in pediatrics. To diagnose EV meningitis, virus isolation in cell cultures is often time consuming and lacks sensitivity to be of clinical relevance. This makes the virus culture results difficult to interpret. The rapid detection of EVs in cerebrospinal fluid (CSF) by molecular diagnostic techniques...

Porcine respiratory disease complex (PRDC) continues to be a significant economic problem for swine producers. Porcine reproductive and respiratory syndrome virus (PRRSV), Swine influenza virus (SIV), Porcine circovirus-2 (PCV-2), Mycoplasma hyopneumoniae, Actinobacillus pleuropneumoniae, and Pasturella multocida are among the most important pathogens contributing to PRDC. Recently, a new gener...