A model for energy conversion in Complex I is proposed that is a conservative expansion of Mitchell's Q-cycle using a simple mechanistic variation of that already established experimentally for Complex III. The model accommodates the following proposals. (1) The large number of flavin and iron-sulfur redox cofactors integral to Complex I form a simple but long electron transfer chain guiding su...

Humic acids (HA) are ubiquitous redox-active compounds of natural aquatic and soil systems. Here we studied the potential of HA as reductants for chlorinated aliphatic pollutants. To avoid artifacts potentially involved when studying chemically reduced HA, we prepared electrochemically reduced soil, aquatic and synthetic HA, and anthrahydroquinone-2,6-disulfonic acid (AHQDS), a model compound f...

Directed evolution methods were developed for Cu-containing nitrite reductase (NiR) from Alcaligenes faecalis S-6. The PCR cloning strategy allows for the efficient production of libraries of 100 000 clones by a modification of a megaprimer-based whole-plasmid synthesis reaction. The high-throughput screen includes colony lift onto a nylon membrane and subsequent lysis of NiR-expressing colonie...

Objective (s): SrCO3 nanoparticles could be used as new biomedical sources in magnetic resonance imaging as a promising noninvasive imaging modality for the preoperative staging of breast cancer and monitoring of tumor response to therapy. The present study aimed to synthesize SrCO3 nanostructures using microwave irradiation in the presence of honey as a green capping agent and reductant. Mater...