BACKGROUND Telomerase plays a role in the immortalization of cells and carcinogenesis. Previous studies have yielded conflicting results on whether human telomerase RNA (hTER) expression differs in nevi, atypical nevi and melanomas using polymerase chain reaction-based telomeric repeat amplification protocol or in situ hybridization assays. The aim of this study was to evaluate human telomerase...

Three hundred fifty-nine cases of stomatitis nicotina were studied in reverse smokers of home made chuttas. One hundred thirty-five biopsies of papular unbilicated lesions of stomatitis nicotina were studied histopathologically. The biopsies were chosen after toluidine blue staining. Mild dysplastic to severe dysplastic changes were seen around the openings of the ducts of the glands. There wer...

Pectins are major components of primary plant cell walls and the seed mucilage of Arabidopsis. Despite progress in the structural elucidation of pectins, only very few enzymes participating in or regulating their synthesis have been identified. A first candidate gene involved in the synthesis of pectinaceous rhamnogalacturonan I is RHM2, a putative plant ortholog to NDP-rhamnose biosynthetic en...

We report an original application of competitive reverse transcription-polymerase chain reaction (RT-PCR) for the quantification of MDR1 mRNA in clinical specimens by simultaneous reverse transcription and PCR amplification of cellular RNA with decreasing amounts of an internal standard. The competitor RNA shares the same MDR1 primer sequences as the cellular mRNA, but yields a different-sized ...

Microglia activation in the spinal cord play a major role pathogenesis of neuropathic pain. The p38 mitogen-activated protein kinase (MAPK) regulates microglia activation. Previously, 2′,3′-dideoxycytidine (ddC), nucleoside reverse transcriptase inhibitor (NRTI), was found to induce mechanical allodynia and cords male female mice. In this study, we investigated MAPK signaling development using ...

To investigate the repair effect of distal-less homeobox 2 transfected bone marrow-derived mesenchymal stem cells on knee cartilage injury. 20 male New Zealand large eared rabbits aged 6 to 7 mo were selected and fed for 1 w undergo injury modeling surgery. Reverse transcription-polymerase chain reaction, western blot hematoxylin-eosin staining used detect these related indicators. Behavioral s...

Purpose: To investigate the role of nuclear receptor-interacting protein 1 (NRIP1) in oxidative stressinduced apoptosis and pyroptosis cataract disease.Methods: Human lens epithelial cells (HLE-B3 cells) were exposed to hydrogen peroxide (H2O2). NRIP1 expression (H2O2)-treated HLE-B3 was determined by western blotting quantitative reverse transcription polymerase chain reaction (qRT-PCR). CCK8 ...

Although miR-5195-3p has been acknowledged for its tumor suppressor role in diverse cancer categories, precise functions and mechanisms concerning melanoma have not comprehensively elucidated. In this study, we employed quantitative reverse transcription PCR, Western blot analysis, immunohistochemistry staining to investigate the expression patterns of poly (rC) binding protein 2 (PCBP2) tissue...

Abstract Bottle gourd [ Lagenaria siceraria (Mol.) Standl.] is one of the most common horticultural and medicinal crops. Genetic transformation a rate-limiting step in unraveling function genes molecular breeding this non-model crop. In study, we established an optimized protocol for using seed germination pouch as growth carrier developed bottle composite plants. Overground explants were inocu...