Abstract Bell's palsy (BP) is an acute, unilateral facial nerve (FNP) that a diagnosis of exclusion, sometimes associated with infectious causes. In this article, we described previously healthy 2-year-old child patient who presented left-sided droop, positive severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) real-time reverse transcription polymerase chain reaction (RT-PCR), SARS-Co...

An mRNA-specific reverse transcription (RT)-PCR primer set spanning the exon junction of a spliced putative terminase gene in the koi herpesvirus (KHV) was developed to detect the replicating stage of the virus. The proposed RT-PCR amplified a target gene from the RNA template, but not from a DNA template extracted from common carp brain (CCB) cells infected with KHV. In addition, the RT-PCR di...

Abstract High-throughput, high-accuracy detection of emerging viruses allows for the control disease outbreaks. Currently, reverse transcription-polymerase chain reaction (RT-PCR) is currently most-widely used technology to diagnose presence SARS-CoV-2. However, RT-PCR requires extraction viral RNA from clinical specimens obtain high sensitivity. Here, we report a method detecting novel coronav...

Duck spleen necrosis disease (DSND) caused by Novel Reovirus (NDRV), is an emerging infectious that causes severely threaten to duck industry. Currently, the popular conventional RT-PCR technique for detecting NDRV time consuming. So, it essential develop a rapid and accurate molecular diagnosis techniques of pathogen purpose effective control disease. In our study, simple, reliable detection m...

Despite routine vaccination programs against Newcastle disease (ND), sporadic cases have occasionally occurred that remain a constant threat to commercial poultry. Ten isolates of Newcastle disease viruses (NDV) from infected broiler chicken cases were obtained from various locations in Fars province during 2009-2011 and genetically analyzed using reverse transcription polymerase chain reaction...

Cowpea mild mottle virus (CPMMV) is a global plant that poses threat to the production and quality of legume crops. Early accurate diagnosis essential for effective managing CPMMV outbreaks. With advancement in isothermal recombinase polymerase amplification lateral flow strips technologies, more rapid sensitive methods have become available detecting this pathogen. In study, we developed rever...