Reversed phase high pressure liquid chromatography (HPLC) solvent systems have been developed for the separation of intermediates in the formation of bile acids and bile acid conjugates from cholesterol. Four different mobile phases (water-methanol, 10 mM acetate buffer (pH 4.37)-methanol, 30 mM trifluoroacetic acid (pH 2.9 with triethylamine)-methanol, and 50 mM potassium phosphate buffer (pH ...

Corn cultured with Aspergillus flavus to produce a high level of aflatoxin was ammoniated at 37 degrees C for 21 days. An extract of the ammoniated corn was separated by thin-layer chromatography, followed by reversed-phase high-pressure liquid chromatography. Examination of the fractions by tandem mass spectrometry led to detection of aflatoxin D1 as a product of the corn ammoniation process.

In this study, high performance liquid chromatography (HPLC) was used to analyze the protodioscin content in Tribulus terrestris samples. They were separated by C18 column, gradient with 0.1 % phosphoric acid solution and acetonitrile as mobile phase flow rate of 0.5 mL/min, column temperature 45 °C, detection wavelength 203 nm. The results showed that has a good linear correlation (r>0.999)...

Ultrafast reversed-phase high-performance liquid chromatographic (HPLC) separations are often needed for analyses related to combinatorial chemistry, studies in liquid chromatography-mass spectrometry, and other applications in which very rapid sample turnaround is paramount. Unfortunately, no consensus exists regarding the best column technology for optimally performing the desired rapid separ...