The aim of the present investigation was to assess and compare the antimicrobial activities of the lemon (Citrus sinensis) peel extract and lemon oil against common food borne pathogens and to study their potential as food bio-preservatives. The antagonistic activity of lemon peel extract and lemon oil was studied against common food borne pathogens like Staphylococcus aureus, Staphylococcus ep...

Solid-state fermentation was carried out using brown rice (BR) and polished rice (PR) as substrates for the production of tempeh using a fungal culture of Rhizopus oligosporus. The rice based tempeh was further analyzed for its nutrient composition like carbohydrate, protein, fat, fiber and ash content. The prepared BR and PR tempeh samples were baked and its sensory qualities were evaluated. O...

GA (glucoamylase) hydrolyses starch and polysaccharides to beta-D-glucose. RoGA (Rhizopus oryzae GA) consists of two functional domains, an N-terminal SBD (starch-binding domain) and a C-terminal catalytic domain, which are connected by an O-glycosylated linker. In the present study, the crystal structures of the SBD from RoGA (RoGACBM21) and the complexes with beta-cyclodextrin (SBD-betaCD) an...

In the presence or absence of acetonitrile CH3CN, the molecular mechanism on the Rhizopus niveus glucoamylse (GA)-catalyzed reaction was studied by the steady-state kinetics for a substrate maltose (G2). At high concentration of G2, the GA-catalyzed reaction was observed not to obey the Michaelis kinetics and was reasonably explained with a mechanism of the substrate-inhibition involving the te...

Our objective was to obtain products of fusion of the filamentous fungus Rhizopus cohnii Rh.c./1 with an increased capacity for lipase biosynthesis in comparison with the original strain. Protoplasts of auxotrophic mutants of the parent strain Rh.c./1 obtained after UV irradiation of the spores were subjected to electrofusion. We found that the largest number of electrofusion products could be ...

Specific Rhizopus microsporus pathovars harbor bacterial endosymbionts (Burkholderia rhizoxinica) for the production of a phytotoxin. Here, we present the draft genome sequences of two R. microsporus strains, one symbiotic (ATCC 62417), and one endosymbiont-free (CBS 344.29). The gene predictions were supported by RNA sequencing (RNA-seq) data. The functional annotation sets the basis for compa...

Mika Murabayashi1, Taku Sugita1, Keiko Hirakawa2,3, Hideto Kuribayashi4, Ikuo Mori1, Kazuki Kubo1, Takashi Horiguchi1, Masayuki Ueda2,5, Yasuo Katayama2,5 and Youkichi Ohno2,3 Takeda Pharmaceutical Company Limited NMR and Small Animal MRI Laboratory, Nippon Medical School Department of Legal Medicine, Nippon Medical School Varian Technologies Japan Ltd. Division of Neurology, Department of Inte...