Chemical Labeling has been employed to attempt to identify the lactate transport protein of rabbit erythrocytes, which have a very high capacity for stereoselective lactate transport. The lactate transport protein catalyzes lactate/proton cotransport (or lactate/hydroxyl exchange) at physiological pH, as demonstrated by uphill proton fluxes induced by lactate gradients. However, lactate/lactate...

Abstract Staphylococcus aureus (S. aureus) is a leading cause of prosthetic joint infection (PJI) typified by biofilm formation. Our laboratory has identified preferential myeloid-derived suppressor cell (MDSC) recruitment as critical mechanism for persistence, MDSCs are main source IL-10. We screened the Nebraska Transposon Mutant Library to identify S. mutants impaired in their ability trigge...

It is generally acknowledged that calcium lactate (ca-lactate) fortification of soymilk to achieve the equivalent level in cow’s milk (1.2 mg/g) causes instability soymilk. In order produce acceptable calcium-enriched with great stability, three different kinds chelating agents were added, namely tripotassium citrate (TPC), trisodium (TSC), and sodium hexametaphosphate (SHMP). this study, addit...

The effect of ethanol on the interrelationship of lactate and glucose metabolism was investigated in eight human volunteers. Lactate and glucose kinetics and intervconversion rates were determined by the sequential administration of L-(+) lactate-U-(14)C and glucose-1-(14)C over an 8 hr period. After a 12 hr fast, the glucose turnover and recycling rates were 94.0 +/-3.8 (SEM) and 13.7 +/-1.1 m...

The cell-to-cell lactate shuttle was introduced in 1984 and has been repeatedly supported by studies using a variety of experimental approaches. Because of its large mass and metabolic capacity, skeletal muscle is probably the major component of the lactate shuttle in terms of both production and consumption. Muscles exercising in a steady state are avid consumers of lactate, using most of the ...

Helicobacter pylori are Gram-negative, spiral-shaped microaerophilic bacteria etiologically related to gastric cancer. Lactate utilization has been implicated although no corresponding genes have been identified in the H. pylori genome. Here, we report that gene products of hp0137-0139 (lldEFG), hp0140-0141 (lctP), and hp1222 (dld) contribute to D- and L-lactate utilization in H. pylori. The th...

UNLABELLED Lactate is a key myocardial energy source. Lactate metabolism is altered in a variety of conditions, such as exercise and diabetes mellitus. However, to our knowledge, noninvasive quantitative measurements of myocardial lactate metabolism have never been performed because of the lack of an adequate radiotracer. In this study we tested L-3-(11)C-lactate ((11)C-lactate) as such a trace...

Once thought to be the consequence of oxygen lack in contracting skeletal muscle, the glycolytic product lactate is formed and utilized continuously under fully aerobic conditions. "Cell-cell" and "intracellular lactate shuttle" concepts describe the roles of lactate in the delivery of oxidative and gluconeogenic substrates, as well as in cell signalling. Examples of cell-cell shuttles include ...