The Asian tiger mosquito, Aedes albopictus, is an invasive vector mosquito of substantial public health concern. large genome size (~1.19–1.28 Gb by cytofluorometric estimates), comprised ~68% repetitive DNA sequences, has made it difficult to produce a high-quality assembly for this species. We constructed high-density linkage map Ae. albopictus based on 111,328 informative SNPs obtained RNAse...

Vascular malformations due to somatic mutation result in abnormal development of endothelial network. The disorder can associate with pain, ulceration, bleeding, recurrent infection, disfigurement, and organ dysfunction. Though surgical interventions including debulking, excision sclerotherapy have shown some success, recurrence disease progression are frequent. Complex cases often not amendabl...

Background The complexity of subcortical brain remains unexplored due to complex neuronal structure and heterogeneity populations in the brain. Raphe regulates multiple physiological events like stress, memory, feeding, sleep, movement, balance, many others. ere we present single cell RNAsec (scRNAseq) analysis raphe tissue from postmortem brains Alzheimer’s disease Braak Stages VI control subj...

Sugarcane smut disease is caused by the biotrophic fungus Sporisorium scitamineum. The disease is characterized by the development of a whip-like structure from the primary meristems, where billions of teliospores are produced. Sugarcane smut also causes tillering and low sucrose and high fiber contents, reducing cane productivity. We investigated the biological events contributing to disease s...

Technological advances in genome-wide transcript analysis, referred to as the transcriptome, using microarrays and deep RNA sequencing methodologies are rapidly extending our understanding of the genetic content of the gammaherpesviruses (γHVs). These vast transcript analyses continue to uncover the complexity of coding transcripts due to alternative splicing, translation initiation and termina...

RNA sequencing approaches to transcriptome analysis require a large amount of input total RNA to yield sufficient mRNA using either poly-A selection or depletion of rRNA. This feature makes it difficult to miniaturize transcriptome analysis for greater efficiency. To address this challenge, we devised and validated a simple procedure for the preparation of whole-transcriptome cDNA libraries fro...