A modified Rose Bengal test (mRB) and an indirect ELISA (iELISA) with Protein G as the conjugate, were evaluated for the diagnosis of Brucella melitensis infection in unvaccinated sheep with a known bacteriological status, and their diagnostic efficacy was compared with that of the standard Rose Bengal (RB) and Complement Fixation (CF) tests used in the current eradication campaign in EU countr...

Photodynamic antimicrobial chemotherapy based on photosensitizers activated by illumination is limited by poor penetration of visible light through skin and tissues. In order to overcome this problem, Rose Bengal was excited in the dark by 28 kHz ultrasound and was applied for inactivation of bacteria. It is demonstrated, for the first time, that the sonodynamic technique is effective for eradi...

Abstract Metal organic frameworks (MOFs) are of great interest for biomedicine due to their high loading capacity various drugs, dyes, and other small molecules. In vivo application MOFs requires sizes nanoparticles colloidal stability. Here we designed the first nano-sized composed bismuth trimesic acid by a rapid microwave-assisted solvothermal method. After coating prepared with polyacrylic ...

The microsnails (Mollusca: Gastropoda), featured by miniscule size (adult shell < 5mm), are little explored among the Indian terrestrial malacofauna. In present study, morphological features, host plant preference and predicted distribution of arboreal microsnail Pupisoma dioscoricola (C. B. Adams, 1845) (Gastropoda: Valloniidae) characterized from West Bengal, India. features were highly co...

A serological survey of brucellosis in cattle and buffalo was performed in 23 States of India. A total of 30,437 bovine samples, comprising 23,284 cattle and 7,153 buffalo (Bubalus bubalis), were screened. The screening initially used the rose bengal plate test: doubtful and positive samples were then titrated in the serum tube agglutination test. The overall prevalence rate of antibodies was 1...

A cross-sectional seroprevalence study was conducted on cattle in the North and Adamawa Regions of Cameroon to investigate the status of bovine brucellosis and identify potential risk factors. The diagnosis was carried out using the Rose Bengal Plate test (RBPT) and indirect ELISA (i-ELISA), while questionnaires were used to evaluate risk factors for bovine brucellosis in cattle. The Bayesian a...

Serum samples from all patients with culture-confirmed brucellosis including those with chronic disease from Kazakhstan tested positive in the serum agglutination test for titers > or = 1:25 and reacted in the Brucella immunoglobulin M/immunoglobulin G lateral flow assay (LFA) confirming the high sensitivity of these assays. The strong reactivity in the LFA observed for the majority (92.1%) of ...

The incidence of a high percentage of negative rose bengal test (~BT~ sera which showed a high titre in the serum agglutmat10n (SAT) and complement fixation tests. (CFf). was noted in certain batches of sera during routme testmg for bo~ine brucellosis. As a similar phe~omenon has not previously been recorded, an investigation was undertaken to establish whether in this case a prozone reaction l...

Sanita-kun(R) Yeasts and Molds (SkYM), a novel dry sheet culture method for rapid enumeration of fungi, has been developed. This re-hydrated plate consists of a unique adhesive sheet, non-woven fabric coated with nutrients, antibiotic, water absorption polymer and uniquely synthesized 2-(2-methoxyphenyl)-3-(4-nitrophenyl)-5-phenyl-tetrazolium chloride for rapid enumeration of yeasts and molds. ...

This study was carried out with a recently developed model of focal cerebral ischemia in the rat based on the photochemical induction of thrombotic stroke using the dye Rose Bengal. We examined the change in the volume of the lesion and brain water content, in separate groups of rats, at different times (1, 4, 24, 72, and 168 h) after the induction of the ischemic lesion. The volume of ischemic...