An efficient method for the rapid separation and purification of polyphenols from artichoke by polyamide column chromatography in combination with high-speed counter-current chromatography (HSCCC) was successfully built. The crude ethanol extracts from dry artichoke were first pre-separated by polyamide column chromatography and divided in two parts as sample 1 and sample 2. Then, the samples w...

Cai W., Gu X., Tang J. (2010): Extraction, purification, and characterisation of the flavonoids from Opuntia milpa alta skin. Czech J. Food Sci., 28: 108–116. The flavonoids contained in Opuntia milpa alta skin were extracted, purified, and identified. Based on single factor experimental results, the extraction parameters were optimised by using orthogonal design and variance analysis. The resu...

Rhagadiolus stellatus Gaertn., a Mediterranean member of the Cichorieae tribe of the Asteraceae family used as a food plant, was analyzed for its spectrum of phenolic compounds. Kaempferol 3-O-β-glucoside 1, kaempferol 3-O-β-rutinoside (nicotiflorin) 2, quercetin 3-O-β-glucoside 3, and luteolin 4 were isolated from the n-butanol layer of a methanolic extract of whole plants of Rh. stellatus of ...

The polyphenol content and antioxidant activity of Nonpareil, Carmel, Butte, Sonora, Fritz, Mission, and Monterey almond cultivars harvested over three seasons in California were examined. LC-MS was employed to quantify 16 flavonoids and two phenolic acids in acidified methanol extracts of almond skins. The 3-year mean polyphenol content of cultivars ranged from 4.0 to 10.7 mg/100 g almonds. Is...

Phenolic composition of different extracts of honeydew melon seeds and their antioxidant activity was determined for the first time. Phenolic compounds were identified using a reversed phase high performance liquid chromatography with diode array detection (HPLC-DAD) method. Results showed the identification of five phenolic compounds in water extract namely gallic acid and its derivative, hydroxyb...

We present our experiment about adding anthocyanins to the daily food of mice. Three kinds of anthocyanins (cyanidin-3-glucoside, cyanidin-3-rutinoside and pelargonidin-3-glucoside) purified from Chinese mulberry (Morus australis Poir) were evaluated for suppressing body weight gain of the male C57BL/6 mice fed with high-fat diet (HFD). The results from a 12-week experiment show that consumptio...

Lemon (Citrus limon) contains various bioactive flavonoids, and prevents obesity and obesity-associated metabolic diseases. We focused on eriocitrin (eriodictyol 7-rutinoside), a powerful antioxidative flavonoid in lemon with lipid-lowering effects in a rat model of high-fat diet. To investigate the mechanism of action of eriocitrin, we conducted feeding experiments on zebrafish with diet-induc...