objective the goal of this study was to prepare and characterize alginate microspheres as an antigen delivery system and adjuvant for immunization against leishmaniasis. materials and methods microspheres were prepared by an emulsification technique and characterized for size, encapsulation efficiency, and release profile of encapsulates. selection of appropriate parameters (viscosity of algina...

conclusions results showed that hcvcp + bcg induced a moderate ctl and mixed th1/th2 immune responses with higher levels of cell proliferation and ifn-γ secretion, indicating that bcg may have a better outcome when formulated in hcvcp-based subunit vaccines. results expression and purification of core protein around the expected size (21 kda) was confirmed by western blotting. the hcvcp + bcg v...

methods mycobacterium tuberculosis standard strain crbip7.11 was cultured at 37°c in loewenstein johnson (lj) media for 3-4 weeks. to confirm the species, standard microbiological and biochemical tests were performed. after preparation of membrane vesicles, the amount of protein in membrane vesicles was measured by sds-page and nanodrop. to analyze the integrity and morphology of extracellular ...

an oil adjuvant vaccine (oav) was prepared from a local strain of pasteurella multocida. strain 6:b was cultured and inactivated by formalin. bacterial pellet was prepared by centrifugation and subsequently adjuvanted by montannide oil isa-70. a dose of prepared vaccine containing 3ml (2mg dry weight/ml) was injected into five calves by im route. animals were bled before and at 24, 90, 150, and...

introduction: long term complications of hepatitis c virus (hcv) infection include fibrosis, cirrhosis, and hepatocellular carcinoma and although the disease is treatable with the newly introduced direct acting antivirals, but factors such as drug resistance, viral genotype and adverse effects can limit the effectiveness of therapy. therefore, development of effective, safe and affordable proph...

A chemically synthesized copy (S-CB7) of a fragment (35 amino acid residues) of type 24 streptococcal M protein was covalently linked to polylysine with carbodiimide and injected subcutaneously into rabbits without adjuvant. Although the primary immune responses as measured by enzyme-linked immunosorbent assays at biweekly intervals were weak, the secondary responses as measured by both enzyme-...

The need to develop a vaccine against schistosomiasis led several researches and our group to investigate proteins from Schistosoma mansoni as vaccine candidates. Sm22.6 is a protein from S. mansoni that shows high identity with Sj22.6 and Sh22.6 (79 and 91%, respectively). These proteins are associated with high levels of IgE and protection to reinfection. Previously, we have shown that Sm22.6...