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Cytoskeleton-associated protein 4 (CKAP4) is a reversibly palmitoylated and phosphorylated transmembrane protein that functions as a high-affinity receptor for antiproliferative factor (APF)-a sialoglycopeptide secreted from bladder epithelial cells of patients with interstitial cystitis (IC). Palmitoylation of CKAP4 by the palmitoyl acyltransferase, DHHC2, is required for its cell surface loca...
Experience-dependent synaptic plasticity refines brain circuits during development. To identify novel protein synthesis-dependent mechanisms contributing to experience-dependent plasticity, we conducted a quantitative proteomic screen of the nascent proteome in response to visual experience in Xenopus optic tectum using bio-orthogonal metabolic labeling (BONCAT). We identified 83 differentially...
We present S factor data obtained from the Coulomb dissociation of 83 MeV/nucleon 8B, and analyze 7Be longitudinal momentum distributions measured at 44 and 81 MeV/nucleon using a potential model, first-order perturbation theory, and dynamical solution of the time-dependent Schrödinger equation. Comparing our results with independent continuum-discretized coupled channels calculations, we study...
Treatment of E. coli ribosomal subunits with 2-iminothiolane coupled with mild ultraviolet irradiation leads to the formation of a large number of RNA-protein cross-links. In the case of the 30S subunit, a number of sites on 16S RNA that are cross-linked to proteins S7 and S8 by this procedure have already been identified (see ref. 6). Here, by using new or modified techniques for the partial d...
Iron (Fe) is an essential mineral nutrient and a metal cofactor required for many proteins and enzymes involved in the processes of DNA synthesis, respiration, and photosynthesis. Iron limitation can have detrimental effects on plant growth and development. Such effects are mediated, at least in part, through the generation of reactive oxygen species (ROS). Thus, plants have evolved a complex r...
Did segmented body plans evolve just once, or repeatedly among the animals? This question has become all the more controversial, now that molecular phylogenetic trees make it clear that the three major clades of segmented animals, vertebrates, annelids and the pan-arthropoda, each lies within a different major clade of the animals. If we are to have any hope of understanding the origins of segm...
against prevalent ESBLand AmpC-producing Enterobacteriaceae from south-east England. J Antimicrob Chemother 2006; 57: 1012–4. 3. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing: Seventeenth Informational Supplement M100-S17. CLSI, Wayne, PA, USA, 2007. 4. Fuchs PC, Barry AL, Thomsberry C et al. Interpretative criteria for temocillin di...
Content Synthetic scheme of phosphoramidite 9b ------------------------------------------------------------------S3 Figure S1 ------------------------------------------------------------------------------------------------------S4 Figure S2 ------------------------------------------------------------------------------------------------------S4 Figure S3 -----------------------------------------...
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