The pathogen group A Streptococcus (GAS) produces a wide spectrum of infections including necrotizing fasciitis (NF). Streptolysin S (SLS) produces the hallmark beta-haemolytic phenotype produced by GAS. The nine-gene GAS locus (sagA-sagI) resembling a bacteriocin biosynthetic operon is necessary and sufficient for SLS production. Using precise, in-frame allelic exchange mutagenesis and single-...

SAGA/TFTC-type multiprotein complexes play important roles in the regulation of transcription. We have investigated the importance of the nuclear positioning of a gene, its transcription and the consequent export of the nascent mRNA. We show that E(y)2 is a subunit of the SAGA/TFTC-type histone acetyl transferase complex in Drosophila and that E(y)2 concentrates at the nuclear periphery. We dem...

The TATA-binding protein (TBP) is critical for transcription by all three nuclear RNA polymerases. In order to identify factors that interact with TBP, the nonnatural photoreactive amino acid rho-benzoyl-phenylalanine (BPA) was substituted onto the surface of Saccharomyces cerevisiae TBP in vivo. Cross-linking of these TBP derivatives in isolated transcription preinitiation complexes or in livi...

# These authors contributed equally to this work. ABSTRACT TAF9 is a TATA binding protein associated factor (TAF) conserved from yeast to humans, and shared by two transcription coactivator complexes, TFIID and SAGA. The essentiality of the TAFs has made it difficult to ascertain their roles in TFIID and SAGA function. Here, we performed a genomic synthetic genetic array analysis using a temper...

Application-level interoperability is defined as the ability of an application to utilize multiple distributed heterogeneous resources. Such interoperability is becoming increasingly important with increasing volumes of data, multiple sources of data as well as resource types. The primary aim of this paper is to understand different ways and levels in which application-level interoperability ca...

The Spt-Ada-Gcn5 acetyltransferase (SAGA) coactivator complex hyperacetylates histone tails in vivo in a manner that depends upon histone 3 lysine 4 trimethylation (H3K4me3), a histone mark enriched at promoters of actively transcribed genes. SAGA contains a separable subcomplex known as the histone acetyltransferase (HAT) module that contains the HAT, Gcn5, bound to Sgf29, Ada2, and Ada3. Sgf2...

Proper gene regulation is vital to the health and development of an organism. Determining the relationship between splicing, transcription, and chromatin structure is vital for understanding gene regulation as a whole. There have been previous studies linking these elements pairwise; however, no evidence exists for a direct link between all three. Recent data shows that splicing components of t...

29th Oct., at 3.30 p.m.: Professor Max Rosenheim, c.b.e., m.d., f.r.c.p. "The Treatment Nephritis". (A combined meeting with the B.M.A. (Exeter and South Western)). Dinner19th Nov., at 8.15 p.m.: Jack Simpson, Esq., m.d., m.r.c.p., "Confusion and Dementia in Elderly". 10th Dec., at 8.15 p.m.: Clinico-pathological Demonstration. f 7th Jan., at 8.15 p.m.: Dr. Sheila Sherlock, m.d., f.r.c.p., "Adv...

A telerobotic test-bed has recently been built between KAIST(Korea) and Saga University(Japan). This paper presents its first research work on investigating the interactive learning performance of human teleoperators. For this purpose, the telerobotic test-bed was re-shaped to a telerobotic golf system. Eleven players were participated, and their interactive performance with the telerobot was c...