Correlative microscopy combines information from light and electron microscopy (LM and EM) into one comprehensive dataset. The “Shuttle & Find” interface from Zeiss was the first easy to use solution for imaging one and the same sample region in different microscopes, i.e. from widefield or confocal LMs to environmental or field emission SEMs (FE-SEM). However, Shuttle & Find is limited to 2D a...

Recently, three-dimensional reconstruction of ultrastructure of the brain has been realized with minimal effort by using scanning electron microscopy (SEM) combined with focused ion beam (FIB) milling (FIB-SEM). Application of immunohistochemical staining in electron microscopy (EM) provides a great advantage in that molecules of interest are specifically localized in ultrastructures. Thus, we ...

Identification of diatom frustules, a vital step during ecological analyses, is greatly influenced by the magnification at which samples are examined. The striking differences between Light Microscopy (LM) and Scanning Electron Microscopy (SEM) reveal inconsistencies in the identification of diatoms at low magnifications. These inconsistencies hinder comparative analyses between data sets colle...

Correlative fractography is a new expression proposed here to describe a new method for the association between scanning electron microscopy (SEM) and light microscopy (LM) for the qualitative and quantitative analysis of fracture surfaces. This article presents a new method involving the fusion of one elevation map obtained by extended depth from focus reconstruction from LM with exactly the s...

Fishing tentacle nematocysts of the hydrozoan, Physalia utriculus, from Hawaiian coastal waters, were examined by light microscopy, as well as by scanning and transmission electron microscopy (SEM and TEM). Hawaiian P. utriculus has been assumed to be conspecific with the Australian morph based on general macroscopic descriptions. Ultrastructural examination using SEM and TEM revealed that the ...

The aqueous leaf extract of Jatropha curcas was used for the synthesis of silver nanoparticles (Jc-AgNps) which were further evaluated for its antibacterial potential against food borne pathogens. J. curcas leaf extract could synthesize stable silver nanoparticles (Zeta potential: -23.4 mV) with absorption band at 430 nm. Fourier transform infrared spectroscopy indicated various biological comp...

This study used optical coherence tomography (OCT) compared to scanning electron microscopy (SEM) and optical microscopy (OM) to evaluate qualitatively crack propagation and final fracture in restorative composite materials - Filtek Z250 and Filtek Z350 (3M ESPE) - with fiber reinforcement after cyclic loading. Samples were made using a split mold. Initially, 3-point bending tests were performe...

Microscopical tests, including polarized light microscopy (PLM), phase contrast microscopy (PCM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used along with X-ray diffraction (XRD) in the characterization and testing of a laboratory-grade “Gooch fiber” asbestos sample. The sample was tested for possible fiber release during typical use as a substrate for ...

Datasets from a slow carbonate vapor diffusion and mineral precipitation protocol for Dictyostelium ECM and cellulose stalks show examples for composite materials obtained by an in vitro approach, which differs substantially from the in vivo approach reported in The Journal of Structural Biology, doi: 10.1016/j.jsb.2016.03.015 [1]. Methods for obtaining the datasets include bright field transmi...