Overexpression of P-glycoprotein, the plasma membrane protein prod uct of the MURI gene, is a major determinant in the development of resistance to a large number of cancer chemotherapeutic agents. A battery of antibodies, including the MURI gene-specific monoclonal antibody mi.\ln C494, is used to evaluate human tissues in clinical multidrug resistance surveillance and modulation trials. In ra...

Phenoxodiol, an isoflavone derivative of genistein with unknown mechanism of action, is currently being evaluated in early human cancer clinical trials. To determine the mechanism of antiproliferative effects of phenoxodiol, we examined its effects in a battery of human cell lines. Although we observed caspase-dependent apoptosis in HN12 cells as early as 24 hours after exposure, clonogenic dea...

Purpose: Metastatic uveal melanoma is resistant to conventional chemotherapy and immunotherapy. In this study, we investigated the responsiveness of uveal melanoma cell lines to IFNs and the hypomethylating agent decitabine. Experimental Design: The uveal melanoma cell lines 92-1, UW-1, OCM-1, and MKT-BR were exposed to varying concentrations of IFN-a, IFN-g, and decitabine, alone and in combin...

In total, 59 single Cys-replacement mutants in helix VII and helix X of the lactose permease of Escherichia coli were subjected to site-directed fluorescence labeling in right-side-out membrane vesicles to complete the testing of Cys accessibility or reactivity. For both helices, accessibility/reactivity is relatively low at the level of the sugar-binding site where the helices are tightly pack...

Grapevine leafroll associated virus 2 (GLRaV 2) is one of the important components in the leafroll disease complex. The coat protein gene of GLRaV 2 was cloned into a protein expression vector pMAL-c2x and the recombinant protein, consisting of the maltose binding protein (MBP) and GLRaV 2 coat protein (CP), was expressed in Escherichia coli. The recombinant MBP-CP was used to raise a high qual...

VLA-2 (also called gpla/IIa on platelets) is a collagen receptor with a unique ot subunit and a subunit common to other adhesion receptors in the VLA/integrin family. Multiple cDNA clones for the human VLA-2 o~ 2 subunit have been selected from a hgtll library by specific antibody screening. The 5,374bp nucleotide sequence encoded for 1,181 amino acids, including a signal peptide of 29 amino ac...

The complexity of the human proteome is greatly expanded by post-translational modifications. New tools capable of recognizing these modifications in a sequence-specific fashion provide a route to purify these modified proteins, to alter protein trafficking, and to visualize signal transduction in real time. Here, we have evolved novel, modification-specific ligands that target phosphorylated I...

The molecular pharmacology of inhalational anesthetics remains poorly understood. Despite accumulating evidence suggesting that neuronal membrane proteins are potential targets of inhaled anesthetics, most currently favored membrane protein targets lack any direct evidence for anesthetic binding. We report herein the location of the binding site for the inhaled anesthetic halothane at the amino...

Purpose: Cytosolic phospholipase A2-a (cPLA2-a) provides intracellular arachidonic acid to supply both cyclooxygenase and lipoxygenase pathways.We aim to determine the expression and activation of cPLA2-a in prostate cancer cell lines and tissue and the effect of targeting cPLA2-a in vitro and in vivo. Experimental Design:The expression of cPLA2-a was determined in prostate cancer cells by reve...

An abundant protein in maize (Zea mays L.) embryos is a storage globulin encoded by the polymorphic Glbl gene. Several Glbl protein size alleles and a null allele have been described. Here we report the isolation and nucleotide sequence analysis of genomic clones corresponding to two Glbl size alleles (Glbl-L and Glbl-S) and to the Glbl-0 null allele. The Glbl-L and Glbl-0 alleles differ from G...