Nonlinear microscopy has become an important tool for imaging biological samples such as neurons. In two photon fluorescence, a fluorophore absorbs two photons at a low energy state, one of its electrons briefly converts to a higher energy state, and it emits a single excited photon at an energy level higher than that of the absorbed photons. In second harmonic generation (SHG), laser light is ...

Two-photon microscopy has revolutionized life sciences by enabling long-term imaging of living preparations in highly scattering tissue while minimizing photodamage. At the same time, commercial two-photon microscopes are expensive and this has prevented the widespread application of this technique to the biological community. As an alternative to commercial systems, we provide an update of our...

Daria A. Smirnova,1 Ilya V. Shadrivov,1 Andrey E. Miroshnichenko,1 Alexander I. Smirnov,2,3 and Yuri S. Kivshar1 1Nonlinear Physics Center, Research School of Physics and Engineering, Australian National University, Canberra ACT 0200, Australia 2Lobachevsky State University, Nizhny Novgorod 603950, Russia 3Institute of Applied Physics, Russian Academy of Sciences, Nizhny Novgorod 603950, Russia...

The application of second-harmonic generation (SHG) microscopy to plant materials has been neglected hitherto even though it would seem to have promise for identification and characterization of biologically and commercially important plant polysaccharides. We find that imaging of cellulose requires rather high laser powers, which are above optimal values for live cell imaging. Starch, however,...

*Corr. Author’s Address: University of Ljubljana, Faculty of Mechanical Engineering, Aškerčeva 6, 1000 Ljubljana, Slovenia, [email protected] 789 CW Fiber Laser for Second Harmonic Generation Novak, V. – Petkovšek, R. – Podobnik, B. – Možina, J. Vid Novak1 – Rok Petkovšek2,* – Boštjan Podobnik1 – Janez Možina2 1 LPKF Laser & Elektronika d.o.o, Slovenia 2 University of Ljubljana, Facult...

Based on density functional theory with the generalized gradient approximation plus on-site Coulomb repulsion method, we study the magnetic-ordering dependence of second-harmonic generation (SHG) in a polar magnet BiCoO(3). The large second-order optical susceptibility, which can reach 3.7x10(-7) esu, exhibits a strong magnetic-ordering dependence, giving rise to magnetic-field controllable SHG...

It is shown that mutual trapping of the fundamental and second-harmonic waves in a dispersive medium with quadratic nonlinearity supports a variety of solitary waves including bright and dark solitons, solitons with trapped radiation, stable bound states of two (or more) solitons. The results are demonstrated for parametric interactions in optical ,$*I materials but can be generalized to other ...

Diffracted surface second-harmonic radiation emerging in several orders has been observed from a periodically modulated monolayer of adsorbed dye molecules. The molecular grating was produced by laser-induced desorption in the field of two crossed beams. An elementary theory is presented that relates the characteristics of the second-harmonic diffraction pattern to the spatially varying propert...

In connection with recent theoretical predictions, enhancement of optical second harmonic generation (SHG) by diffractive coupling to the silver surface-plasmon polariton (SPP) mode is shown experimentally to be maximized on a biperiodic corrugated surface. Optimized first-order diffractive coupling of incident radiation to the SPP maximizes resonance enhancement of the surface-localized electr...