A relatively simple method for obtaining ultrathin, frozen sections for electron microscopy has been developed. Tissues, cultured cells, and bacteria may be employed. They are fixed in 1.25-4% glutaraldehyde for 1-4 hr, are washed overnight in buffer at 3 degrees C, and are embedded in 20% thiolated gelatin or pure gelatin. Before sectioning they are partially dehydrated in 50% glycerol, frozen...

A simple reproducible protocol for detecting multiple copy human genes and viral DNA in routine formalin fixed paraffin embedded tonsil and brain, by in situ hybridisation with biotinylated probes, is described. The protocol consists of digestion of formalin fixed paraffin sections, with 0 4% pepsin in 0 01 M hydrochloric acid for one hour at 37°C, followed by hybridisation with biotinylated pr...

We discuss present predictions for the total γγ and γp cross-sections, highlighting why predictions differ. We present results from the Eikonal Minijet Model and improved predictions based on soft gluon resummation.

The top quark is the latest quark of the Standard Model (SM) that was discovered, discovery that was made in 1995 by the CDF and D0 collaborations [1]. It is the “youngest” quark of the SM and many of its properties remain to be studied in detail. Since it is also the heaviest elementary particle known so far, its status in the SM is special. Indeed, the top quark is 40 times heavier than its w...