Helicobacter pylori has been isolated from the human stomach with media containing only minimal selective agents. However, current research on the transmission and sources of infection requires more selective media due to the higher numbers of contaminants in environmental, oral, and fecal samples. The objective of this study was to develop and evaluate detection techniques that are sufficientl...

This case report illustrates the need for regular review of culture methods and maintenance of thorough attempts at diagnosis prior to treatment of gonorrhoea. Vancomycin-sensitive gonococcal infection was discovered by repeated tests and by using a non-selective medium (locally). The support of the Gonococcus Reference Unit confirmed the findings. The observations are discussed in laboratory, ...

Adenosine is released during tissue injury, ischemia and tumor growth, and promotes angiogenesis. Because mast cells accumulate in the proximity of new blood vessel development, we examined if they may contribute to adenosine-induced angiogenesis. We found that HMC-1 human mast cells express A2A, A2B, and A3 adenosine receptors. The adenosine agonist NECA (100 micromol/L) increased interleukin-...

For the isolation and cultivation of Legionella pneumophila from tap water in hospitals, we compared different media and selection techniques. A second part of the study compared the L. pneumophila yields from different water samples at identical sites. A total of 210 water samples (500 ml each) were collected from two selected sites in each of 21 hospitals. Warm water samples were collected af...

Engberg et al. (2) compared selective media and filtration for the efficient isolation of campylobacteria from stools. Since 1977, we have routinely isolated campylobacters from the diarrhetic stools of pediatric patients at the Red Cross Children’s Hospital in Cape Town, South Africa. In 1990, the use of antibiotic-containing plates was discontinued, and the Cape Town protocol, the first to co...