In this study, quantitive characteristics and chemical composition of in roach (Rutilus rutilus caspicus) sperm were investigated. Sperm traits included sperm movement duration, percentage of motile spermatozoa, sperm density, spermatocrit and sperm volume. Some seminal plasma characteristics (sodium, potassium, calcium, magnesium and chloride) were investigated. In addition, some metabolites o...

Lactoferrin with a molecular mass of 80 kDa was purified from equine seminal plasma by heparin-Agarose affinity chromatography and Sephacryl S-200 gel filtration. Purified lactoferrin was found to be highly homogeneous on the bases of its migration as a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and of the monospecificity of rabbit antibodies to the purified protei...

The ability of stallion seminal plasma to modify phagocytosis of spermatozoa and Streptococcus zooepidemicus was examined. Phagocytosis was monitored indirectly as the H2O2 produced by peripheral blood leukocytes after addition of spermatozoa or bacteria. Hydrogen peroxide production after addition of ejaculated spermatozoa was greater (P less than 0.01) than after addition of epididymal sperm....

This study was undertaken to investigate whether there is relationship between seminal plasma transferrin and seminal parameters which included sperm count, motility and morphology. The study included 100 male subjects in the age group of 23-41 yrs including 7 proven fertility, 6 post-vasectomised and 87 subjects were of idiopathic infertility. Estimation of seminal plasma transferrin concentra...

A major secretory protein of the human epididymis that is taken up by maturing spermatozoa is homologous to the leukocyte antigen CD52. The epididymis was shown to be the sole source of CD52 in seminal fluid, since CD52 could be detected in seminal plasma from sperm-containing ejaculates and not in ejaculates of vasectomized patients by Western blot analysis. The glycoprotein is not expressed i...

BACKGROUND Prostaglandin E(2) (PGE(2)) has been shown to modulate angiogenesis and tumour progression via the E-series prostanoid-2 (EP2) receptor. Endometrial adenocarcinomas may be exposed to endogenous PGE(2) and exogenous PGE(2), present at high concentration in seminal plasma. METHODS This study investigated fibroblast growth factor 2 (FGF2) mRNA expression and cell signalling in respons...

Annexin A5 (anxA5) is abundantly present in seminal plasma, however, its endogenous function in seminal plasma is not known. Recently, we demonstrated that folic acid and zinc sulfate intervention increased sperm count. To explore the involvement of anxA5 in human (sub)fertility, we measured anxA5 concentrations in seminal plasma, using sandwich ELISA, before and after folic acid (5 mg/day) and...

The effect of seminal plasma, as well as some seminal plasma fractions, on the heterogeneity and viability of frozen-thawed ram spermatozoa was studied. Fresh and frozen sperm samples were simultaneously assessed by a routine semen-quality assay and centrifugal countercurrent distribution (CCCD) in an aqueous two-phase system analysis. All samples used for this study were obtained from Salz ram...