PURPOSE To clarify the cellular origin of extranodal marginal-zone B-cell lymphoma (EZML) of the mucosa-associated lymphoid tissue (MALT) type in ocular adnexa, the somatic mutation was analyzed in the immunoglobulin heavy-chain variable region (VH) gene. METHODS Eight cases of EZML in the orbit and four in the conjunctiva were studied. The VH genes were amplified by a seminested PCR and sequ...

Rotavirus gastroenteritis is a major cause of severe dehydrating diarrhea in children worldwide. Rotavirus G and P genotyping is essential for epidemiological surveillance and for better formulation of candidate rotavirus vaccines. Out of 862 diarrheal stool samples collected from hospitalized children aged < 2 years during February 2005 - March 2007, 318 (36.9%) were positive for rotavirus by ...

Human rhinoviruses (RVs), comprising three species (A, B, and C) of the genus Enterovirus, are responsible for the majority of upper respiratory tract infections and are associated with severe lower respiratory tract illnesses such as pneumonia and asthma exacerbations. High genetic diversity and continuous identification of new types necessitate regular updating of the diagnostic assays for th...

1154 Emerging Infectious Diseases • www.cdc.gov/eid • Vol. 11, No. 7, July 2005 PanBio Pty. Ltd., Brisbane, Australia). The same acute-phase serum was tested for flavivirus RNA by seminested reverse transcription–polymerase chain reaction (RT-PCR) by using flavivirus consensus primers PF1S and PF2R as previously described (1) in conjunction with the sense primer PF3S (GCIATHTGGTAYATGTGGYT). Att...

To the Editor: In recent years, several novel respiratory viruses have been identified. These include human metapneumovirus (HMPV) (1), severe acute respiratory syndrome– associated coronavirus (2), human coronavirus (HCoV) NL63 (3,4), HCoV HKU1 (5), and, most recently, human bocavirus (HBoV) (6). The latter belongs to the Parvoviridae family and is most closely related to bovine parvovirus and...

BACKGROUND: Group A bovine rotavirus (BRV-A) is one of the most important causes of gastroenteritis and diarrhea in newborn calves. OBJECTIVES: Major types of BRV-A in Tehran, Alborz and Qazvin were detected in this study. METHODS: A total 125 fecal samples of calves showing clinical signs of diarrhea were collected from 26 industrial dairy farms located in the provinces of Tehran, Alborz and Q...

BACKGROUND The genotype of hepatitis C virus (HCV) is a predictor of antiviral therapeutic response. We describe an approach for HCV genotype determination by real-time PCR and melting curve analysis. METHODS After automated nucleic acid extraction, we used reverse transcription-PCR in a block cycler to amplify nucleotides 6-329 of the 5'-untranslated region of HCV. The product was further am...

background: group a bovine rotavirus (brv-a) is one of the most important causes of gastroenteritis and diarrhea in newborn calves. objectives: major types of brv-a in tehran, alborz and qazvin were detected in this study. methods: a total 125 fecal samples of calves showing clinical signs of diarrhea were collected from 26 industrial dairy farms located in the provinces of tehran, alborz and q...

PCR products are detected most commonly using postamplification agarose gel electrophoresis and/or DNA hybridization. For reducing time in detection and confirmation, a number of investigators have reported methods to simplify or even eliminate the need to perform postamplification assays. For example, molybodate (2) or some intercalating agents (3) have been used to measure the degree of DNA a...